ADAM15 Antibody (007) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89546

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Sandwich ELISA Detection, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007 expressed in HEK293

Format

Azide and BSA Free
View all ADAM15 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ADAM15 (Accession#: NP_997074.1; Met 1-Thr 696).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ADAM15 Antibody (007) - Azide and BSA Free

Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546]

Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546]

Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546] - Staining of human heart showing blood vessel staining (red arrow) (1:200).
Flow Cytometry: ADAM15 Antibody (007) [NBP2-89546]

Flow Cytometry: ADAM15 Antibody (007) [NBP2-89546]

Flow Cytometry: ADAM15 Antibody (007) [NBP2-89546] - Profile of anti-ADAM15 reactivity on MCF-7 cells.
Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546]

Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546]

Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546] - Staining of human lymphonode showing membrane staining of endothelial cells (red arrow) (1:2000).
ADAM15 Antibody (007) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: ADAM15 Antibody (007) [NBP2-89546] -

Immunocytochemistry/Immunofluorescence: ADAM15 Antibody (007) [NBP2-89546] - Staining of Human ADAM15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89546 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.

Applications for ADAM15 Antibody (007) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry

1:1000-1:4000

Immunohistochemistry-Paraffin

1:1000-1:4000

Sandwich ELISA Detection

1:1000-1:10000
Application Notes
This antibody will detect Human ADAM15 in ELISA pair set (Catalog: # NBP2-79482). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP2-89548).

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADAM15

ADAM15 is encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Long Name

A Disintegrin and Metalloprotease-like Domain 15

Alternate Names

MDC15, Metargidin

Gene Symbol

ADAM15

Additional ADAM15 Products

Product Documents for ADAM15 Antibody (007) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ADAM15 Antibody (007) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for ADAM15 Antibody (007) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review ADAM15 Antibody (007) - Azide and BSA Free and earn rewards!

Have you used ADAM15 Antibody (007) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies