ADE2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90235

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat, Chinese Hamster

Cited:

Human, Hamster - Cricetulus (Chinese Hamster)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ADE2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ETAFIAPQCEMIPIEWVCRRIATGSFLKRNPGVKEGYKFYPPKVELFFKDDANNDPQWSEEQLIAAKFCFAGLLIGQTEVDIMS

Reactivity Notes

Chinese Hamster reactivity reported in scientific literature (PMID: 23394948).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ADE2 Antibody - BSA Free

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Analysis in human liver and skeletal muscle tissues using NBP1-90235 antibody. Corresponding PAICS RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]

Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
Simple Western: ADE2 Antibody [NBP1-90235]

Simple Western: ADE2 Antibody [NBP1-90235]

Simple Western: ADE2 Antibody [NBP1-90235] - Electropherogram image(s) of corresponding Simple Western lane view. ADE2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
ADE2 Antibody - BSA Free Western Blot: ADE2 Antibody - BSA Free [NBP1-90235]

Western Blot: ADE2 Antibody - BSA Free [NBP1-90235]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Western Blot: ADE2 Antibody [NBP1-90235]

Western Blot: ADE2 Antibody [NBP1-90235]

Western Blot: ADE2 Antibody [NBP1-90235] - Analysis using Anti-PAICS antibody NBP1-90235 (A) shows similar pattern to independent antibody NBP2-58966 (B).

Applications for ADE2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 47, 50 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADE2

The ADE2 gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine bio

Alternate Names

ADE2, AIR carboxylase, AIRCADE2H1, DKFZp781N1372, MGC1343, MGC5024, multifunctional protein ADE2, PAIS, phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazolesuccinocarboxamide synthetase, SAICAR synthetase

Entrez Gene IDs

10606 (Human)

Gene Symbol

PAICS

UniProt

P22234 (Human)

Additional ADE2 Products

Product Documents for ADE2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ADE2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ADE2 Antibody - BSA Free

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Protocols

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