Adenosine A1R Antibody - BSA Free

Novus Biologicals | Catalog # NB300-549

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Adenosine A1R Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 18948751). Rat from customer review.

Localization

Cell Membrane

Specificity

Detects Adenosine Receptor A1. This does not detect other AR subtypes.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenosine A1R Antibody - BSA Free

Western Blot: Adenosine A1R AntibodyBSA Free [NB300-549]

Western Blot: Adenosine A1R AntibodyBSA Free [NB300-549]

Western Blot: Adenosine A1R Antibody [NB300-549] - Analysis was performed on whole cell extracts (30 ug lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5).
Immunocytochemistry/ Immunofluorescence: Adenosine A1R Antibody - BSA Free [NB300-549]

Immunocytochemistry/ Immunofluorescence: Adenosine A1R Antibody - BSA Free [NB300-549]

Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on 70% confluent log phase HeLa cells.
Immunohistochemistry-Paraffin: Adenosine A1R Antibody - BSA Free [NB300-549]

Immunohistochemistry-Paraffin: Adenosine A1R Antibody - BSA Free [NB300-549]

Immunohistochemistry-Paraffin: Adenosine A1R Antibody [NB300-549] - Analysis showing positive staining in the cytoplasm of Rat brain tissue (right) compared with a negative control in the absence of primary antibody (left).
Flow Cytometry: Adenosine A1R Antibody - BSA Free [NB300-549]

Flow Cytometry: Adenosine A1R Antibody - BSA Free [NB300-549]

Flow Cytometry: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (PA1041A, red histogram) or with rabbit isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Immunocytochemistry/ Immunofluorescence: Adenosine A1R Antibody - BSA Free [NB300-549]

Immunocytochemistry/ Immunofluorescence: Adenosine A1R Antibody - BSA Free [NB300-549]

Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NB300-549] - Dissociated rat hippocampal culture. Image courtesy of Janosch Heller, University College London.

Applications for Adenosine A1R Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

3 - 5 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

2 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500
Application Notes
Adenosine A1R antibody validated for ICC/IF from a verified customer review. Use in IHC- Frozen reported in scientific literature (PMID: 24016034).

Reviewed Applications

Read 1 review rated 4 using NB300-549 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Adenosine A1R

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission.The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.

Long Name

Adenosine A1 Receptor

Alternate Names

Adenosine A1 R, ADORA1, RDC7

Entrez Gene IDs

29290 (Rat)

Gene Symbol

ADORA1

UniProt

P25099 (Rat)

Additional Adenosine A1R Products

Product Documents for Adenosine A1R Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Adenosine A1R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Adenosine A1R Antibody - BSA Free

Customer Reviews for Adenosine A1R Antibody - BSA Free (1)

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  • Name: Bryan Tinsley
    Application: Immunocytochemistry
    Sample Tested:
    Species: Rat
    Verified Customer | Posted 08/27/2014
    ICC on dissociated rat hippocampal culture.
    Adenosine A1R Antibody - BSA Free NB300-549

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