Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80562

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Canine, Guinea Pig, Rabbit, Sheep

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 7F6-G5-A2

Format

Azide and BSA Free
View all Adenosine A2aR Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human Adenosine A2a R. [UniProt# P29274]

Epitope

Epitope mapped to SQPLPGER in the third intracellular loop (PMID 9822147).

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 29791204). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free

Western Blot: Adenosine A2aR Antibody (7F6-G5-A2)Azide and BSA Free [NBP2-80562]

Western Blot: Adenosine A2aR Antibody (7F6-G5-A2)Azide and BSA Free [NBP2-80562]

Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Immunohistochemistry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Immunohistochemistry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - Staining of human brain (putamen), antibody at 5 ug/mL. Image from the standard format of this antibody.
Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - Using the PE direct conjugate an intracellular stain was performed on SH-SY5Y cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control NB600-986PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin (PE).
Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:100 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:400 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors. Image from the standard format of this antibody.
Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - An intracellular stain was performed on SH-SY5Y with Adenosine A2a R Antibody (7F6-G5-A2) NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B). Image from the standard format of this antibody.
Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - An intracellular stain was performed on U-87 MG cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.
Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]

Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.

Applications for Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 - 5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100. Use reported in scientific literature (PMID 25836920)

Immunohistochemistry

1 - 5 ug/ml

Immunohistochemistry-Frozen

reported in scientific literature (PMID 9822147)

Immunohistochemistry-Paraffin

1 - 5 ug/ml

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenosine A2aR

This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Long Name

Adenosine A2A Receptor

Alternate Names

A2aR, Adenosine A2a R, ADORA2A, RDC8

Gene Symbol

ADORA2A

Additional Adenosine A2aR Products

Product Documents for Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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