Adenosine A2b R Antibody
Novus Biologicals | Catalog # NB600-797
![Immunocytochemistry/ Immunofluorescence: Adenosine A2b R Antibody [NB600-797]](https://resources.rndsystems.com/images/products/Adenosine-A2b-R-Antibody-Immunocytochemistry-Immunofluorescence-NB600-797-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Adenosine A2b R Antibody [NB600-797]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Rabbit
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry, Western Blot, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
Peptide with sequence CQADVKSGNGQ corresponding to C-Terminus according to NP_000667.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Adenosine A2b R Antibody
Immunocytochemistry/ Immunofluorescence: Adenosine A2b R Antibody [NB600-797]
Immunocytochemistry/Immunofluorescence: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml, showing cytoplasmic staining. The nuclear stain is DAPI (blue)![Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NB600-797]](https://resources.rndsystems.com/images/products/Adenosine-A2b-R-Antibody-Immunohistochemistry-Paraffin-NB600-797-img0002.jpg "Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NB600-797]")
Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NB600-797]
Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NB600-797] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.![Flow Cytometry: Adenosine A2b R Antibody [NB600-797]](https://resources.rndsystems.com/images/products/Adenosine-A2b-R-Antibody-Flow-Cytometry-NB600-797-img0003.jpg "Flow Cytometry: Adenosine A2b R Antibody [NB600-797]")
Flow Cytometry: Adenosine A2b R Antibody [NB600-797]
Flow Cytometry: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line)![Immunocytochemistry/ Immunofluorescence: Adenosine A2b R Antibody [NB600-797]](https://resources.rndsystems.com/images/products/Adenosine-A2b-R-Antibody-Immunocytochemistry-Immunofluorescence-NB600-797-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Adenosine A2b R Antibody [NB600-797]")
Immunocytochemistry/ Immunofluorescence: Adenosine A2b R Antibody [NB600-797]
Immunocytochemistry/Immunofluorescence: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml, showing cytoplasmic staining. The nuclear stain is DAPI (blue)Applications for Adenosine A2b R Antibody
Application
Recommended Usage
Flow Cytometry
10ug/ml
Immunocytochemistry/ Immunofluorescence
2-4ug/ml
Immunohistochemistry
2 - 4 ug/ml
Immunohistochemistry-Paraffin
3 ug/ml
Peptide ELISA
detection limit 1:64000
Application Notes
IHC: In paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells.
Flow Cytometry: Flow cytometric analysis of Kelly cells.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells.
Flow Cytometry: Flow cytometric analysis of Kelly cells.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Adenosine A2b R
Long Name
Adenosine A2b Receptor
Alternate Names
Adenosine A2bR, ADORA2B
Entrez Gene IDs
136 (Human)
Gene Symbol
ADORA2B
UniProt
Additional Adenosine A2b R Products
Product Documents for Adenosine A2b R Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Adenosine A2b R Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Adenosine A2b R Antibody
Powered by Bioz
Customer Reviews for Adenosine A2b R Antibody
There are currently no reviews for this product. Be the first to review Adenosine A2b R Antibody and earn rewards!
Have you used Adenosine A2b R Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...