Adenovirus Hexon Antibody - BSA Free
Novus Biologicals | Catalog # NB600-1386
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Key Product Details
Species Reactivity
Virus
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Simple Western
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
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Product Specifications
Immunogen
Gradient purified, disrupted native adenovirus type 5 virions
Reactivity Notes
Cross-reacts with Adenovirus. Does not react with Para 1-3, Influenza A and B, or RSV.
Localization
Major coat protein of adenoviruses.
Specificity
Recognizes numerous Adeno proteins including hexon protein, and reacts with Adenovirus types 1, 2, 3, 5, 6, 7a, 8, 31, 40 & 41. Broad reactivity with other Adenovirus types is expected. Goat anti Adenovirus antibody does not cross-react with Parainfluenza types 1-3, Influenza A & B, RSV, hMPV and Rhinovirus type 16. Goat anti Adenovirus antibody does not react with uninfected Hep-2, LLCMK2 and Vero cells. Adenoviruses are 80-110 nm diameter, non-enveloped icosahedral viruses that can cause respiratory illness, infantile gastroenteritis and other mild disorders such as conjunctivitis. Features of Adenovirus have made it a useful tool for gene transfection and expression in mammalian cells.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Adenovirus Hexon Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Simple Western
1:100
Western Blot
1:100
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in A549, HEK 293, antibody dilution of 1:100
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
4.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Adenovirus Hexon
Alternate Names
Hexon protein; Late protein 2; PII
Additional Adenovirus Hexon Products
Product Documents for Adenovirus Hexon Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Adenovirus Hexon Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Adenovirus Hexon Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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