Adenylate Kinase 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87401

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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat, Rhesus Macaque

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Adenylate Kinase 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGS

Reactivity Notes

Rhesus macaque reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenylate Kinase 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining in human fallopian tube and small intestine tissues using anti-AK1 antibody. Corresponding AK1 RNA-seq data are presented for the same tissues.
Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401]

Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401]

Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunocytochemistry/Immunofluorescence: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining of human small intestine shows low expression as expected.
Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401]

Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401]

Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining of human fallopian tube shows high expression.

Applications for Adenylate Kinase 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-87401 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenylate Kinase 1

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Long Name

Adenylate kinase isoenzyme 1

Alternate Names

AK 1, AK1, EC 2.7.4.3, EC 2.7.4.6, Myokinase

Entrez Gene IDs

203 (Human)

Gene Symbol

AK1

UniProt

P00568 (Human)

Additional Adenylate Kinase 1 Products

Product Documents for Adenylate Kinase 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Adenylate Kinase 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Adenylate Kinase 1 Antibody - BSA Free (1)

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  • Adenylate Kinase 1 Antibody
    Name: Addie Luo
    Application: Western Blot
    Sample Tested: Adult lung
    Species: Rhesus Macaque
    Verified Customer | Posted 08/13/2021
    expression of Adenylate kinase 1 in rhesus macaque lung (lane 1:lung)
    Adenylate Kinase 1 Antibody - BSA Free NBP1-87401
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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