ADNP Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-97747

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ADNP Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human ADNP fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ADNP Antibody - BSA Free

Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.

Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.
Immunocytochemistry/ Immunofluorescence: ADNP Antibody [NBP2-97747]

Immunocytochemistry/ Immunofluorescence: ADNP Antibody [NBP2-97747]

Immunocytochemistry/Immunofluorescence: ADNP Antibody [NBP2-97747] - Immunofluorescence staining of ADNP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ADNP polyclonal antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red). Positive staining was localized to Nucleus.
Immunoprecipitation: ADNP Antibody [NBP2-97747]

Immunoprecipitation: ADNP Antibody [NBP2-97747]

Immunoprecipitation: ADNP Antibody [NBP2-97747] - ADNP was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate. 4 ul anti-ADNP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ADNP rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa. Observed band size: 150 kDa.
ADNP Antibody

Detection of ADNP by Immunoprecipitation.

DMS 53 cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of ADNP Antibody (Catalog # NBP2-97747) pre-coupled to Dynabeads Protein A. Immunoprecipitated ADNP was detected in Western Blot with a Rabbit ADNP antibody used at 1/500. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
ADNP Antibody

Western Blot Shows ADNP Specificity Using Knockdown Cell Line.

Western blot shows lysates of DMS 53 parental cell line and ADNP knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with ADNP Antibody (Catalog # NBP2-97747) followed by HRP-conjugated secondary antibody. A specific band was detected for ADNP at approximately 135 kDa (as indicated) in the parental DMS 53 cell line, but is significantly reduced in the DMS 53 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Applications for ADNP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunoprecipitation

5-10 uL/mg of lysate

Knockdown Validated

Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADNP

The activity-dependent neuroprotective protein (hADNP) gene is frequently amplified in many neoplasias, including breast, bladder, ovarian, pancreatic, and colon cancers. hADNP mRNA is abundantly expressed in distinct normal tissues, and high expression levels were encountered in malignant cells. hADNP is implicated in maintaining cell survival, perhaps through modulation of p53.

Long Name

Activity-dependent Neuroprotective Protein

Alternate Names

ADNP1

Gene Symbol

ADNP

Additional ADNP Products

Product Documents for ADNP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ADNP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ADNP Antibody - BSA Free

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Protocols

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