ADNP Antibody - BSA Free

Detection of ADNP by Immunoprecipitation.

DMS 53 cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of ADNP Antibody (Catalog # NBP2-97747) pre-coupled to Dynabeads Protein A. Immunoprecipitated ADNP was detected in Western Blot with a Rabbit ADNP antibody used at 1/500. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot Shows ADNP Specificity Using Knockdown Cell Line.

Western blot shows lysates of DMS 53 parental cell line and ADNP knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with ADNP Antibody (Catalog # NBP2-97747) followed by HRP-conjugated secondary antibody. A specific band was detected for ADNP at approximately 135 kDa (as indicated) in the parental DMS 53 cell line, but is significantly reduced in the DMS 53 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.