Adropin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-26387

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Adropin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human Adropin (within residues 10-60). [Swiss-Prot# Q6UWT2]

Reactivity Notes

88% sequence identity with bovine protein.

Localization

secreted.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adropin Antibody - BSA Free

Western Blot: Adropin AntibodyBSA Free [NBP1-26387]

Western Blot: Adropin AntibodyBSA Free [NBP1-26387]

Western Blot: Adropin Antibody [NBP1-26387] - Total protein from Human and Mouse brain was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-Adropin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Western Blot: Adropin AntibodyBSA Free [NBP1-26387]

Western Blot: Adropin AntibodyBSA Free [NBP1-26387]

Western Blot: Adropin Antibody [NBP1-26387] - Western blot on Adropin overexpression lysate.
Adropin Antibody - BSA Free

Western Blot: Adropin Antibody - BSA Free [NBP1-26387] -

Metabolic molecules in macrophage with adropin treatment. (A) Variations of key molecules involved in glycol–lipid metabolism. (B) Akt, mTOR, and AMPK levels of adropin-treated macrophages. (C) Detection of the above metabolism molecules in ENHO−/− macrophages. (D) Akt, mTOR, and AMPK levels of ENHO−/− macrophages. (E) Effect of ROS depletion or CPT1 alpha inhibition on inflammasome activation. Each experiment was repeated at least twice Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37904094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Adropin Antibody - BSA Free

Western Blot: Adropin Antibody - BSA Free [NBP1-26387] -

Ectopic adropin expression inhibits tumor growth in vivo. (A) Presence of adropin in the indicated cell lines. (B) Ectopic adropin expression in MC38 cells by lentivirus transfection. (C) In vivo tumors formed by MC38 and MC38-ENHO cells. (D) Weights of tumors. Detection of macrophages (E) and CD8+ T cells (F) in tumors. (G) Phenotypic analysis of tumor-infiltrated macrophages. The experiment was repeated twice. ns, no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37904094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Adropin Antibody - BSA Free

Flow Cytometry: Adropin Antibody - BSA Free [NBP1-26387] -

Correlational analysis of adropin level with local macrophages in CRC. Correlation study of adropin with CD68, ARG1, or iNOS in tumor foci (A) or stromal cells (B). (C) Comparison of infiltrated macrophages between high and low ENHO transcriptions. (D) Correlation study of GPR19 with adropin in foci and stroma. (E) Correlation study of GPR19 with CD68, ARG1, or iNOS. Double staining of adropin and CD68, ARG1, iNOS, or PD-L1 (F) and statistics (G). Double staining of GPR19 and CD68, ARG1, iNOS, or PD-L1 (H) and statistics (I). ns, no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37904094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Adropin Antibody - BSA Free

Western Blot: Adropin Antibody - BSA Free [NBP1-26387] -

Macrophages in ENHO−/− mice. (A) Adropin variations in macrophages after the treatment of LPS/IFN gamma, IL-4, IL-10, or TGF-beta 1. (B) Macrophage subsets in ENHO−/− mice. (C) CD86 and CD206 expression on WT or KO macrophages treated by LPS/IFN gamma or IL-4. (D) Inhibitory effects of WT or KO macrophages on CD8+ T cells. Inflammasome-associated molecules (E) and effector molecules (F) of WT or KO macrophages. The experiment was carried-out three times. ns, no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37904094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Adropin Antibody - BSA Free

Application
Recommended Usage

Western Blot

2 ug/ml
Application Notes
A band can be seen at 15 kDa in Western Blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.11 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adropin

Adropin (energy homeostasis-associated protein, ENHO) is a peptide hormone encoded by gene ENHO and its unique ORF generates a 76-residue long secretary peptide which is highly conserved in eutherian (placental) mammals. Adropin is abundantly expressed in liver/brain, and in mouse, hepatic expression as well as circulating adropin concentrations exhibits rapid regulation by fasting (inhibition) and refeeding (stimulation), suggesting regulation through metabolic status signals. Mouse obesity models further confirmed that adropin is involved in metabolic homeostasis and cardiovascular function. Diet-induced as well as genetically induced obesity has been found to be associated with decreased hepatic adropin expression and circulating adropin concentrations. Adropin-knockout mice exhibit increased adiposity and fasting triglycerides (TG), hepatic steatosis, insulin resistance, and increased propensity for impaired glucose tolerance with diet-induced obesity.

Alternate Names

C9orf165, ENHO, UNQ470

Entrez Gene IDs

375704 (Human)

Gene Symbol

ENHO

UniProt

Q6UWT2

Additional Adropin Products

Product Documents for Adropin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Adropin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Glucose Homeostasis
Obesity

Citations for Adropin Antibody - BSA Free

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Protocols

View specific protocols for Adropin Antibody - BSA Free (NBP1-26387):

Adropin Antibody:
Procedure Guide for NBP1-26387 - Adropin Antibody
Western Blot Protocol
1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer
apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of
proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations
and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10
minutes each.
7. Dilute the rabbit anti-Adropin primary antibody (NBP1-26387) in blocking buffer and incubate 1 hour at room
temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10
minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers
instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as
required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided
it does not interfere with antibody-antigen binding.
(c) 2009 Novus Biologicals - Adropin Antibody - Page 1

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