AF4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-28728
![Western Blot: AF4 Antibody [NBP1-28728]](https://resources.rndsystems.com/images/products/AF4-Antibody-Western-Blot-NBP1-28728-img0001.jpg "Western Blot: AF4 Antibody [NBP1-28728]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human proto-oncogene AF4 using the numbering given in entry NP_005926.1 (GeneID 4299).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AF4 Antibody - BSA Free
Western Blot: AF4 Antibody [NBP1-28728]
Western Blot: AF4 Antibody [NBP1-28728] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF4 antibody NBP1-28728 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. AF4 was also immunoprecipitated by rabbit anti-AF4 antibody NBP1-28705, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).![Immunohistochemistry-Paraffin: AF4 Antibody [NBP1-28728]](https://resources.rndsystems.com/images/products/AF4-Antibody-Immunohistochemistry-NBP1-28728-img0002.jpg "Immunohistochemistry-Paraffin: AF4 Antibody [NBP1-28728]")
Immunohistochemistry-Paraffin: AF4 Antibody [NBP1-28728]
Immunohistochemistry-Paraffin: AF4 Antibody [NBP1-28728] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- AF4 used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for AF4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Western Blot
1:2000-1:10000
Application Notes
Not recommended for immunoprecipitation. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: AF4
Alternate Names
AF-4, AF4/FMR2 family member 1, AF4/FMR2 family, member 1, AF4myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 2, ALL1-fused gene from chromosome 4 protein, FEL, MLLT2MGC134969, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 2, PBM1, pre-B-cell monocytic leukemia partner 1, Protein AF-4, Protein FEL, Proto-oncogene AF4
Entrez Gene IDs
4299 (Human)
Gene Symbol
AFF1
UniProt
Additional AF4 Products
Product Documents for AF4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AF4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for AF4 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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