African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25368

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Lateral Flow Assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1286 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of African Swine Fever Virus p54. The exact sequence is proprietary.

Reactivity Notes

African Swine Fever Virus

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] -

Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-25368 [HL1286]) Detection: ASFV p54 antibody (NBP3-25366 [HL1218]) Samples (80 ng) : 1. ASFV p54 (ECD) protein 2. Lysis buffer
African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

ELISA: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] -

ELISA: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] - Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below. Capture: ASFV p54 antibody [HL1286] (NBP3-25368) (5 ug/mL) Detection: HRP-conjugated ASFV p54 antibody [HL1287] (NBP3-25369) (1 ug/mL) Please notice that NBP3-25369 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25368. Please contact us for custom HRP-conjugated antibody.
African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

ELISA: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] -

ELISA: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] - Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below. Capture: ASFV p54 antibody [HL1287] (NBP3-25369) (5 ug/mL) Detection: HRP-conjugated ASFV p54 antibody [HL1286] (NBP3-25368) (1 ug/mL) Please notice that NBP3-25368 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25369. Please contact us for custom HRP-conjugated antibody.
African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] -

Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-25368 [HL1286]) Detection: ASFV p54 antibody (NBP3-25370 [HL1289]) Samples (80 ng) : 1. ASFV p54 (ECD) protein 2. Lysis buffer
African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

ELISA: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] -

ELISA: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length ASFV p54, His Tag using antibodies as below. Capture: ASFV p54 antibody [HL1286] (NBP3-25368) (5 ug/mL) Detection: HRP-conjugated ASFV p54 antibody [HL1289] (NBP3-25370) (1 ug/mL) Please notice that NBP3-25370 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25368. Please contact us for custom HRP-conjugated antibody.
African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

Western Blot: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] -

Western Blot: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1286] (NBP3-25368) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

ELISA: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] -

ELISA: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] - Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below. Capture: ASFV p54 antibody [HL1286] (NBP3-25368) (5 ug/mL) Detection: HRP-conjugated ASFV p54 antibody [HL1289] (NBP3-25370) (1 ug/mL) Please notice that NBP3-25370 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25368. Please contact us for custom HRP-conjugated antibody.
African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] -

Immunocytochemistry/Immunofluorescence: African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free [NBP3-25368] - ASFV p54 antibody [HL1286] detects ASFV p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASFV p54 stained by ASFV p54 antibody [HL1286] (NBP3-25368) diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:5000-1:100000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: African Swine Fever Virus p54

Alternate Names

ASFV p54, Ba71V-126, E183L, Envelope protein p54, Structural protein p54

Gene Symbol

E183L

Additional African Swine Fever Virus p54 Products

Product Documents for African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for African Swine Fever Virus p54 Antibody (HL1286) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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