AG-3/AGR3 Antibody (AGR3.1) - BSA Free
Novus Biologicals | Catalog # NBP1-45004
![Western Blot: AG-3/AGR3 Antibody (AGR3.1)BSA Free [NBP1-45004]](https://resources.rndsystems.com/images/products/AG-3-AGR3-Antibody-AGR3-1-Western-Blot-NBP1-45004-img0002.jpg "Western Blot: AG-3/AGR3 Antibody (AGR3.1)BSA Free [NBP1-45004]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # AGR3.1
Format
BSA Free
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Product Specifications
Immunogen
Purified human AGR3 protein
Specificity
The antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa); a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AG-3/AGR3 Antibody (AGR3.1) - BSA Free
Western Blot: AG-3/AGR3 Antibody (AGR3.1)BSA Free [NBP1-45004]
Western Blot: AG-3/AGR3 Antibody (AGR3.1) [NBP1-45004] - Analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.![Immunohistochemistry: AG-3/AGR3 Antibody (AGR3.1) - BSA Free [NBP1-45004]](https://resources.rndsystems.com/images/products/AG-3-AGR3-Antibody-AGR3-1-Immunohistochemistry-NBP1-45004-img0001.jpg "Immunohistochemistry: AG-3/AGR3 Antibody (AGR3.1) - BSA Free [NBP1-45004]")
Immunohistochemistry: AG-3/AGR3 Antibody (AGR3.1) - BSA Free [NBP1-45004]
Immunohistochemistry: AG-3/AGR3 Antibody (AGR3.1) [NBP1-45004] - Staining of AGR3 with mouse monoclonal AGR3.1 in paraffin-embedded sections of breast carcinoma.
Western Blot: Mouse Monoclonal AG-3/AGR3 Antibody (AGR3.1) [NBP1-45004] -
Western Blot: Mouse Monoclonal AG-3/AGR3 Antibody (AGR3.1) [NBP1-45004] - Western blotting analysis was performed on whole cell extracts (HOT lysis buffer) of MCF-7, HeLa, and Jurkat cell lines, mixed and heated (100C, 5 min) with reducing SDS-loading buffer. Samples were resolved using 15% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody AGR3.1 (1 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.AGR3 molecules were detected at ~ 14 kDa in MCF-7 cell line.Applications for AG-3/AGR3 Antibody (AGR3.1) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 ug/ml
Immunohistochemistry
1 ug/ml
Immunohistochemistry-Frozen
1 ug/ml
Immunohistochemistry-Paraffin
1 ug/ml
Western Blot
1 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: AG-3
Long Name
Anterior Gradient 3 Homolog
Alternate Names
AG3, AGR3, BCMP11, PDIA18
Gene Symbol
AGR3
Additional AG-3 Products
Product Documents for AG-3/AGR3 Antibody (AGR3.1) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AG-3/AGR3 Antibody (AGR3.1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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