AGAP1 Antibody (OTI4E8) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-72357
![Immunohistochemistry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]](https://resources.rndsystems.com/images/products/AGAP1-Antibody-OTI4E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72357-img0004.jpg "Immunohistochemistry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4E8
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human AGAP1(NP_055729) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for AGAP1 Antibody (OTI4E8) - Azide and BSA Free
Immunohistochemistry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]
Immunohistochemistry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357] - Staining of paraffin-embedded Human tonsil using anti-AGAP1 mouse monoclonal antibody.![Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]](https://resources.rndsystems.com/images/products/AGAP1-Antibody-OTI4E8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72357-img0003.jpg "Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]")
Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]
Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357] - Analysis of Jurkat cells, using anti-AGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).![Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]](https://resources.rndsystems.com/images/products/AGAP1-Antibody-OTI4E8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72357-img0001.jpg "Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]")
Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]
Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGAP1.Applications for AGAP1 Antibody (OTI4E8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: AGAP1
Alternate Names
AGAP-1, ANK repeat and PH domains 1, ArfGAP with GTPase domain, ankyrin repeat and PH domain 1, centaurin, gamma 2, Centaurin-gamma-2, CENTG2, Cnt-g2, GTP-binding and GTPase-activating protein 1, KIAA1099arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1, MGC71657
Gene Symbol
AGAP1
Additional AGAP1 Products
Product Documents for AGAP1 Antibody (OTI4E8) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AGAP1 Antibody (OTI4E8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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