AGAP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77194

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AGAP2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE. The immunogen is located within the last 50 amino acids of PIKE. Amino Acid Squence: PRRRSSAASVGRADAP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%)

Specificity

Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AGAP2 Antibody - BSA Free

Western Blot: AGAP2 AntibodyBSA Free [NBP1-77194]

Western Blot: AGAP2 AntibodyBSA Free [NBP1-77194]

Western Blot: AGAP2 Antibody [NBP1-77194] - Analysis of AGAP2 in mouse brain cell lysates with anti-AGAP2 (CT) using this antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
AGAP2 Antibody - BSA Free

Immunohistochemistry: AGAP2 Antibody - BSA Free [NBP1-77194] -

Immunohistochemistry: AGAP2 Antibody - BSA Free [NBP1-77194] - Immunohistochemistry of AGAP2 in mouse brain tissue with AGAP2 antibody at 10 ug/mL.

Applications for AGAP2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AGAP2

Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

Alternate Names

AGAP-2, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2, arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, centaurin, gamma 1, Centaurin-gamma-1, CENTG1, Cnt-g1, FLJ16430, GGAP2, GTP-binding and GTPase activating protein 2, GTP-binding and GTPase-activating protein 2, KIAA0167, Phosphatidylinositol-3-kinase enhancer, phosphoinositide 3-kinase enhancer, PIKEArf GAP with GTP-binding protein-like, ANK repeat and PH domains 2

Gene Symbol

AGAP2

UniProt

AAM97540 (Human)

Additional AGAP2 Products

Product Documents for AGAP2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for AGAP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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