AGER Antibody (JF0975)

Novus Biologicals | Catalog # NBP2-67095

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF0975 expressed in HEK293
View all RAGE/AGER Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human AGER aa 350-390. (SwissProt: Q15109 Human; SwissProt: Q62151 Mouse; SwissProt: Q63495 Rat)

Localization

Cell membrane, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AGER Antibody (JF0975)

Western Blot: AGER Antibody (JF0975) [NBP2-67095]

Western Blot: AGER Antibody (JF0975) [NBP2-67095]

Western Blot: AGER Antibody (JF0975) [NBP2-67095] - Analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095]

Immunocytochemistry/ Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095]

Immunocytochemistry/Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095] - Staining RAGE in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095]

Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095]

Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095] - Mouse lung cancer tissue stained with AGER antibody. IHC-P image submitted by a verified customer review.
Flow Cytometry: AGER Antibody (JF0975) [NBP2-67095]

Flow Cytometry: AGER Antibody (JF0975) [NBP2-67095]

Flow Cytometry: AGER Antibody (JF0975) [NBP2-67095] - Analysis of Jurkat cells with RAGE antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095]

Immunocytochemistry/ Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095]

Immunocytochemistry/Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095] - Staining RAGE in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095]

Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095]

Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095] - Analysis of paraffin-embedded mouse lung tissue using anti-RAGE antibody. Counter stained with hematoxylin.

Applications for AGER Antibody (JF0975)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-67095 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAGE/AGER

AGER encodes a member of the immunoglobulin superfamily of cell surface molecules. It is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products. The gene lies within the major histocompatibility complex (MHC) class III region on chromosome 6. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Long Name

Receptor for Advanced Glycation End Products

Alternate Names

AGER, SCARJ1

Gene Symbol

AGER

Additional RAGE/AGER Products

Product Documents for AGER Antibody (JF0975)

Certificate of Analysis

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Product Specific Notices for AGER Antibody (JF0975)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for AGER Antibody (JF0975) (1)

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Customer Images

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  • AGER Antibody (JF0975)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Lung cancer tissue
    Species: Mouse
    Verified Customer | Posted 12/07/2021
    Lung cancer tissue
    AGER Antibody (JF0975) NBP2-67095

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Protocols

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FAQs

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