AGPAT6 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-48199
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
1-acylglycerol-3-phosphate O-acyltransferase 6(lysophosphatidic acid acyltransferase, zeta). (Uniprot# Q86UL3)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AGPAT6 Antibody - BSA Free
Immunoprecipitation: AGPAT6 Antibody - BSA Free [NBP3-48199] -
Immunoprecipitation: AGPAT6 Antibody - BSA Free [NBP3-48199] - IP Result of anti-AGPAT6 (IP:, 4ug; Detection: 1:1000) with mouse brain tissue lysate 2640ug.
Western Blot: AGPAT6 Antibody - BSA Free [NBP3-48199] -
Western Blot: AGPAT6 Antibody - BSA Free [NBP3-48199] - Mouse testis tissue were subjected to SDS PAGE followed by western blot with (AGPAT6 antibody) at dilution of 1:400
Immunohistochemistry: AGPAT6 Antibody - BSA Free [NBP3-48199] -
Immunohistochemistry: AGPAT6 Antibody - BSA Free [NBP3-48199] - Immunohistochemistry of paraffin-embedded human testis using (AGPAT6 antibody) at dilution of 1:100Applications for AGPAT6 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:20-1:200
Immunoprecipitation
1:500-1:5000
Western Blot
1:500-1:5000
Application Notes
Theoretical Molecular Weight is 48-50 kDa
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 50% Glycerol, pH (7.3)
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AGPAT6
Agpat6 deficiency leads to a reduction in body weight and resistance to both diet-induced and genetically induced obesity. This reduced body weight is associated with increased energy expenditure, reduced triglyceride accumulation in BAT and WAT, reduced white adipocyte size, and lack of adipose tissue in the subdermal region. AGPAT6 fills a unique role in determining triglyceride content and composition in adipose tissue and liver that cannot be compensated by other members of the Agpat family.
Alternate Names
1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acidacyltransferase, zeta), Acyl-CoA:glycerol-3-phosphate acyltransferase 4, DKFZp586M1819,1-acyl-sn-glycerol-3-phosphate acyltransferase zeta, EC 2.3.1.15, glycerol-3-phosphate acyltransferase 4,1-AGP acyltransferase 6, glycerol-3-phosphate acyltransferase 6,1-AGPAT 6, GPAT4,1-acylglycerol-3-phosphate O-acyltransferase 6, LPAAT-zetaLPAATZ, Lysophosphatidic acid acyltransferase zeta, testis spermatogenesis apoptosis-related protein 7, TSARG7
Gene Symbol
AGPAT6
Additional AGPAT6 Products
Product Documents for AGPAT6 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AGPAT6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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