AGXT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89200

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AGXT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HPMTKDPGGHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGS

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26609667).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AGXT Antibody - BSA Free

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Analysis in human liver and pancreas tissues. Corresponding AGXT RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Staining of human endometrium, fallopian tube, skin and testis using Anti-NEK1 antibody (A) NBP1-89200 shows similar protein distribution across tissues to independent antibody NBP2-34198 (B).
Western Blot: AGXT Antibody [NBP1-89200]

Western Blot: AGXT Antibody [NBP1-89200]

Western Blot: AGXT Antibody [NBP1-89200] - Analysis in human liver tissue.
Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Staining of human liver shows strong granular positivity in cytoplasm in hepatocytes.
Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200]

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Simple Western: AGXT Antibody [NBP1-89200]

Simple Western: AGXT Antibody [NBP1-89200]

Simple Western: AGXT Antibody [NBP1-89200] - Simple Western lane view shows a specific band for AGXT in 0.2 mg/ml of Liver (left), HepG2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: AGXT Antibody [NBP1-89200]

Simple Western: AGXT Antibody [NBP1-89200]

Simple Western: AGXT Antibody [NBP1-89200] - Electropherogram image(s) of corresponding Simple Western lane view. AGXT antibody was used at 1:20 dilution on Liver lysate(s).
Simple Western: AGXT Antibody [NBP1-89200]

Simple Western: AGXT Antibody [NBP1-89200]

Simple Western: AGXT Antibody [NBP1-89200] - Electropherogram image(s) of corresponding Simple Western Lane view. AGXT antibody was used at 1:20 dilution on HepG2 lysate(s).

Applications for AGXT Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000-1:2500

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Liver, HepG2, separated by Size, antibody dilution of 1:20, apparent MW was 47 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AGXT

AGXT, also known as Serine--pyruvate aminotransferase, is 43kDa and 392 amino acids long. AGXT is localized to the peroxisome and is only expressed in the liver. AGXT is implicated in glyoxylate detoxification and mutations in the AGXT gene have been linked with type I primary hyperoxaluria. AGXT has been shown to have interactions with AGXT2, PEX5, ALAS2, 14-3-3 epsilon and Synphilin-1.

Alternate Names

AGT1L-alanine: glyoxylate aminotransferase 1, AGThepatic peroxisomal alanine:glyoxylate aminotransferase, AGXT1, alanine-glyoxylate aminotransferase, Alanine--glyoxylate aminotransferase, EC 2.6.1.44, EC 2.6.1.51, PH1, serine:pyruvate aminotransferase, SPATserine-pyruvate aminotransferase, SPTserine--pyruvate aminotransferase, TLH6

Entrez Gene IDs

189 (Human)

Gene Symbol

AGXT

UniProt

P21549 (Human)

Additional AGXT Products

Product Documents for AGXT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for AGXT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AGXT Antibody - BSA Free

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Protocols

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