AHCYL1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-98585

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AHCYL1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human AHCYL1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for AHCYL1 Antibody - BSA Free

Western Blot: AHCYL1 Antibody [NBP2-98585]

Western Blot: AHCYL1 Antibody [NBP2-98585]

Western Blot: AHCYL1 Antibody [NBP2-98585] - Anti-AHCYL1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 59 kDa
Immunohistochemistry-Paraffin: AHCYL1 Antibody [NBP2-98585]

Immunohistochemistry-Paraffin: AHCYL1 Antibody [NBP2-98585]

Immunohistochemistry-Paraffin: AHCYL1 Antibody [NBP2-98585] - Immunochemical staining of human AHCYL1 in human pancreas with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: AHCYL1 Antibody [NBP2-98585]

Immunohistochemistry-Paraffin: AHCYL1 Antibody [NBP2-98585]

Immunohistochemistry-Paraffin: AHCYL1 Antibody [NBP2-98585] - Immunochemical staining of human AHCYL1 in human duodenum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: AHCYL1 Antibody [NBP2-98585]

Immunoprecipitation: AHCYL1 Antibody [NBP2-98585]

Immunoprecipitation: AHCYL1 Antibody [NBP2-98585] - AHCYL1 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 4 ul anti-AHCYL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-AHCYL1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 59 kDa
AHCYL1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: AHCYL1 Antibody [NBP2-98585] -

Immunocytochemistry/Immunofluorescence: AHCYL1 Antibody [NBP2-98585] - Immunofluorescence staining of AHCYL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98585 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for AHCYL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AHCYL1

adenosylhomocysteinase-like 1;PRO0233;XPVKONA;S-adenosyl homocysteine hydrolase homolog;IRBIT;AdoHcyase 2;S-adenosylhomocysteine hydrolase-like 1;DC-expressed AHCY-like molecule;DCAL;EC 3.3.1.1;dendritic cell expressed AHCY-like protein;S-adenosylhomocysteine hydrolase-like protein 1;inositol 1,4,5-trisphosphate receptor-binding protein;S-adenosyl-L-homocysteine hydrolase 2;

Alternate Names

adenosylhomocysteinase-like 1, AdoHcyase 2, DCAL, DC-expressed AHCY-like molecule, dendritic cell expressed AHCY-like protein, inositol 14,5-trisphosphate receptor-binding protein, IRBIT, PRO0233, putative adenosylhomocysteinase 2, S-adenosyl homocysteine hydrolase homolog, S-adenosylhomocysteine hydrolase-like 1, S-adenosylhomocysteine hydrolase-like protein 1, S-adenosyl-L-homocysteine hydrolase 2, XPVKONAEC 3.3.1.1

Gene Symbol

AHCYL1

Additional AHCYL1 Products

Product Documents for AHCYL1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for AHCYL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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