AHNAK Antibody (EM-09) - BSA Free
Novus Biologicals | Catalog # NBP2-62204
![Immunocytochemistry/ Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204]](https://resources.rndsystems.com/images/products/AHNAK-Antibody-EM-09-Immunocytochemistry-Immunofluorescence-NBP2-62204-img0002.jpg "Immunocytochemistry/ Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204]")
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # EM-09
Format
BSA Free
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Product Specifications
Immunogen
Bacterially expressed fragment of N-terminal domain of human AHNAK1.
Specificity
The mouse monoclonal antibody EM-09 reacts with AHNAK, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
700 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AHNAK Antibody (EM-09) - BSA Free
Immunocytochemistry/ Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204]
Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).![Immunohistochemistry-Frozen: AHNAK Antibody (EM-09) [NBP2-62204]](https://resources.rndsystems.com/images/products/AHNAK-Antibody-EM-09-Immunohistochemistry-Frozen-NBP2-62204-img0003.jpg "Immunohistochemistry-Frozen: AHNAK Antibody (EM-09) [NBP2-62204]")
Immunohistochemistry-Frozen: AHNAK Antibody (EM-09) [NBP2-62204]
Immunohistochemistry-Frozen: AHNAK Antibody (EM-09) [NBP2-62204] - Staining (frozen sections) of murine tongue by anti-AHNAK1 antibody (EM-09; red). Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).![Immunocytochemistry/ Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204]](https://resources.rndsystems.com/images/products/AHNAK-Antibody-EM-09-Immunocytochemistry-Immunofluorescence-NBP2-62204-img0001.jpg "Immunocytochemistry/ Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204]")
Immunocytochemistry/ Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204]
Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Applications for AHNAK Antibody (EM-09) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: AHNAK
Alternate Names
AHNAK nucleoprotein, AHNAK nucleoprotein (desmoyokin), AHNAK-related, AHNAKRS, desmoyokin, MGC5395, neuroblast differentiation-associated protein AHNAK, PM227
Gene Symbol
AHNAK
Additional AHNAK Products
Product Documents for AHNAK Antibody (EM-09) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AHNAK Antibody (EM-09) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for AHNAK Antibody (EM-09) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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