AHSA1 Antibody (OTI1D2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71608

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D2

Format

Azide and BSA Free
View all AHSA1 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-203 of human AHSA1 (NP_036243) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AHSA1 Antibody (OTI1D2) - Azide and BSA Free

Western Blot: AHSA1 Antibody (OTI1D2)Azide and BSA Free [NBP2-71608]

Western Blot: AHSA1 Antibody (OTI1D2)Azide and BSA Free [NBP2-71608]

Western Blot: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AHSA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiAHSA1.(1:2000)
Immunohistochemistry: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608]

Immunohistochemistry: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608]

Immunohistochemistry: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using antiAHSA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)
Immunohistochemistry: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608]

Immunohistochemistry: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608]

Immunohistochemistry: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AHSA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150)
Immunohistochemistry: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608]

Immunohistochemistry: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608]

Immunohistochemistry: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-AHSA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)
Knockout Validated: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608]

Western Blot: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608]

Western Blot: AHSA1 Antibody (OTI1D2) - Azide and BSA Free [NBP2-71608] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and AHSA1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-AHSA1 monoclonal antibody. Then the blotted membrane was stripped and re-probed with antiHSP90AB1antibody as a loading control.(1:500)

Applications for AHSA1 Antibody (OTI1D2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AHSA1

Cochaperone that stimulates HSP90 ATPase activity. May affect a step in the endoplasmic reticulum to Golgi trafficking

Alternate Names

activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast), C14orf3, chromosome 14 open reading frame 3, p38AHA1

Gene Symbol

AHSA1

Additional AHSA1 Products

Product Documents for AHSA1 Antibody (OTI1D2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AHSA1 Antibody (OTI1D2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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