Aiolos/IKZF3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24495

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Aiolos/IKZF3 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 350-400 of human IKZF3 was used as the immunogen.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: canine (88%), mouse (88%), rat (88%), equine (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aiolos/IKZF3 Antibody - BSA Free

Western Blot: Aiolos/IKZF3 AntibodyBSA Free [NBP2-24495]

Western Blot: Aiolos/IKZF3 AntibodyBSA Free [NBP2-24495]

Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Total protein from Jurkat cells and human tonsil was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-IkZF3 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Aiolos/IKZF3 Antibody - BSA Free [NBP2-24495]

Immunocytochemistry/ Immunofluorescence: Aiolos/IKZF3 Antibody - BSA Free [NBP2-24495]

Immunocytochemistry/Immunofluorescence: Aiolos/IKZF3 Antibody [NBP2-24495] - IKZF3 antibody was tested in Jurkat cells at a 1:40 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.
Western Blot: Aiolos/IKZF3 AntibodyBSA Free [NBP2-24495]

Western Blot: Aiolos/IKZF3 AntibodyBSA Free [NBP2-24495]

Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Analysis of IKZF3 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 0.25 ug/mL.
Aiolos/IKZF3 Antibody - BSA Free

Western Blot: Aiolos/IKZF3 Antibody - BSA Free [NBP2-24495] -

LEN treatment decreased CK1-alpha levels in hTERT-hMSCshTERT-hMSCs were seeded in T75 flasks and cultured in RPMI 10% FBS, in presence of LEN (0.1 and 1 μM) or DMSO, for 5 days. At the end of culture period, cell pellets were collected and analyzed by Western Blotting for Cereblon (A), Ikaros (B), Aiolos (B), IRF4 (B), CK1-alpha (C) and p62 (D). beta -actin was used as internal control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28881793), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Aiolos/IKZF3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

reported in scientific literature (PMID 34207079)

Western Blot

0.25 - 1.0 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aiolos/IKZF3

IKZF3 encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Long Name

DNA-binding Protein Aiolos

Alternate Names

AIO, IKZF3, ZNFN1A3

Gene Symbol

IKZF3

UniProt

Q9UKT9

Additional Aiolos/IKZF3 Products

Product Documents for Aiolos/IKZF3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Aiolos/IKZF3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aiolos/IKZF3 Antibody - BSA Free

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