AK2 Antibody (AT7E7) - BSA Free
Novus Biologicals | Catalog # NBP2-50575
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # AT7E7
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human AK2 (1-239aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for AK2 Antibody (AT7E7) - BSA Free
Western Blot: AK2 Antibody (AT7E7)BSA Free [NBP2-50575]
Western Blot: AK2 Antibody (AT7E7) [NBP2-50575] - The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: NIH/3T3 cell lysateImmunocytochemistry/ Immunofluorescence: AK2 Antibody (AT7E7) - BSA Free [NBP2-50575]
Immunocytochemistry/Immunofluorescence: AK2 Antibody (AT7E7) [NBP2-50575] - Analysis of AK2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK2 antibody with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Western Blot: AK2 Antibody (AT7E7)BSA Free [NBP2-50575]
Western Blot: AK2 Antibody (AT7E7) [NBP2-50575] - The Tissue lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Kidney tissue lysate Lane 2.: Liver tissue lysateApplications for AK2 Antibody (AT7E7) - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AK2
Long Name
Adenylate kinase 2, mitochondrial
Alternate Names
ADK2, AK 2
Gene Symbol
AK2
Additional AK2 Products
Product Documents for AK2 Antibody (AT7E7) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AK2 Antibody (AT7E7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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