AKAP9 Antibody

Novus Biologicals | Catalog # NB100-1517

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AKAP9 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SGSTTQFHAGMR corresponding to C-Terminus according to NP_005742.4, NP_671714.1.

Reactivity Notes

Rat reactivity reported in scientific literature. (PMID: 21177871). Expected from sequence similarity: Mouse

Specificity

This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

450 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AKAP9 Antibody

Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody [NB100-1517]

Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody [NB100-1517]

Immunocytochemistry/Immunofluorescence: AKAP9 Antibody [NB100-1517] - analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody [NB100-1517]

Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody [NB100-1517]

Immunocytochemistry/Immunofluorescence: AKAP9 Antibody [NB100-1517] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

Applications for AKAP9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10ug/ml

Peptide ELISA

1:32000

Western Blot

1-3 ug/ml
Application Notes
Western blot: This product has been successfully used by a customer, showing a band at approx 450kDa in lysates of cell line HepG2 (calculated MW of 453kDa according to NP_005742.4).
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells.

Reviewed Applications

Read 1 review rated 4 using NB100-1517 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AKAP9

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in many isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3.

Alternate Names

A kinase (PRKA) anchor protein (yotiao) 9, AKAP 350, AKAP 450, AKAP350A-kinase anchor protein 450 kDa, AKAP450A-kinase anchor protein 350 kDa, AKAP-9, Centrosome- and Golgi-localized PKN-associated protein, CG-NAPAKAP 120-like protein, hgAKAP 350, HYPERION, KIAA0803A-kinase anchor protein 9, MU-RMS-40.16A, PRKA9AKAP9-BRAF fusion protein, Protein hyperion, protein kinase A anchoring protein 9, Protein kinase A-anchoring protein 9, Protein yotiao, YOTIAOkinase N-associated protein

Entrez Gene IDs

10142 (Human)

Gene Symbol

AKAP9

UniProt

Q99996 (Human)

Additional AKAP9 Products

Product Documents for AKAP9 Antibody

Certificate of Analysis

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Product Specific Notices for AKAP9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for AKAP9 Antibody (1)

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  • Name: Marta Szaszak
    Application: Western Blot
    Sample Tested: neonatal cardiac myocytes
    Species: Rat
    Verified Customer | Posted 10/04/2011

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