AKAP95/AKAP8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90196

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AKAP95/AKAP8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVP

Marker

Nucleus without Nucleoli Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKAP95/AKAP8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AKAP95/AKAP8 Antibody [NBP1-90196]

Immunocytochemistry/ Immunofluorescence: AKAP95/AKAP8 Antibody [NBP1-90196]

Immunocytochemistry/Immunofluorescence: AKAP95/AKAP8 Antibody [NBP1-90196] - Staining of human cell line U-251 MG shows localization to nucleoplasm.
AKAP95/AKAP8 Antibody - BSA Free Immunohistochemistry: AKAP95/AKAP8 Antibody - BSA Free [NBP1-90196]

Immunohistochemistry: AKAP95/AKAP8 Antibody - BSA Free [NBP1-90196]

Staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.
AKAP95/AKAP8 Antibody - BSA Free Immunohistochemistry: AKAP95/AKAP8 Antibody - BSA Free [NBP1-90196]

Immunohistochemistry: AKAP95/AKAP8 Antibody - BSA Free [NBP1-90196]

Staining of human skeletal muscle shows strong nuclear positivity in myocytes.
AKAP95/AKAP8 Antibody - BSA Free Immunohistochemistry: AKAP95/AKAP8 Antibody - BSA Free [NBP1-90196]

Immunohistochemistry: AKAP95/AKAP8 Antibody - BSA Free [NBP1-90196]

Staining of human duodenum shows strong nuclear positivity in glandular cells and lymphoid cells.
AKAP95/AKAP8 Antibody - BSA Free Immunohistochemistry: AKAP95/AKAP8 Antibody - BSA Free [NBP1-90196]

Immunohistochemistry: AKAP95/AKAP8 Antibody - BSA Free [NBP1-90196]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Western Blot: AKAP95/AKAP8 Antibody [NBP1-90196]

Western Blot: AKAP95/AKAP8 Antibody [NBP1-90196]

Western Blot: AKAP95/AKAP8 Antibody [NBP1-90196] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
AKAP95/AKAP8 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: AKAP95/AKAP8 Antibody - BSA Free [NBP1-90196]

Chromatin Immunoprecipitation-exo-Seq: AKAP95/AKAP8 Antibody - BSA Free [NBP1-90196]

ChIP-Exo-Seq composite graph for Anti-AKAP8 (NBP1-90196) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for AKAP95/AKAP8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKAP95/AKAP8

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Alternate Names

A kinase (PRKA) anchor protein 8, AKAP-8, AKAP-95 AKAP 95, A-kinase anchor protein 8, A-kinase anchor protein 95 kDa, A-kinase anchor protein, 95kDa, DKFZp586B1222

Gene Symbol

AKAP8

Additional AKAP95/AKAP8 Products

Product Documents for AKAP95/AKAP8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for AKAP95/AKAP8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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