ALDH1L1 Antibody (2E7) - BSA Free

Novus Biologicals | Catalog # NBP2-50033

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry Free-Floating

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E7

Format

BSA Free
View all ALDH1L1 Primary Antibodies »

Product Specifications

Immunogen

Amino acids 402-902 of human ALDH1L1. [UniProt# O75891]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH1L1 Antibody (2E7) - BSA Free

Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033]

Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033]

Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of different tissue and cell lysates using mouse mAb to ALDH1L1, NBP2-50033, dilution 1:5,000 in green: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney,[5] lung, [6] brain, and [7] spinal cord; mouse tissue lysates: [8] brain, and [9] spinal cord; cell lysates: [10] NIH-3T3, and [11] HEK293. The band at 100kDa mark corresponds to ALDH1L1 protein.
Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033]

Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033]

Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to aldehyde dehydrogenase 1L1 (ALDH1L1), NBP2-50033, dilution 1:1,000 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. NBP2-50033 antibody produces cytoplasmic staining of glial cells, while the GFAP antibody labels the intermediate filament cytoskeleton in astrocytes and other glial cells. Some astrocytic cells express both ALDH1L1 and GFAP and appear yellow.
Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033]

Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033]

Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Neuron-glia cell mixed cultures stained with NBP2-50033 (red) and chicken polyclonal antibody against Vimentin (NB300-223, green). Blue is a DNA stain. NBP2-50033 stains astrocytic cell bodies and processes. The fibroblastic cells contain only Vimentin and so are green, while astrocytes contain either Vimentin and ALDH1L1, so appearing golden, or predominantly ALDH1L1, in which case they appear red.
ALDH1L1 Antibody (2E7) - BSA Free

Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (2E7) - BSA Free [NBP2-50033] -

AKT2 is expressed in astroglia. (A) Representative image of AKT2 (red) costaining with astrocyte markers ALDH1L1 (green) and glial fibrillary acidic protein (GFAP, blue) in the HPC and CTX of coronal mouse brain sections. AKT2 is present throughout these brain regions, with strong immunoreactivity in the molecular layer of the DG. (B) Higher magnification of the rectangular area in (A) shows ALDH1L1 expression throughout cortical layers I-VI like AKT2, whereas GFAP is largely absent from the CTX and displays greater immunoreactivity in the HPC. (C) Higher magnification of the white square in (B) confirms that AKT2 colocalizes with ALDH1L1, while little to no GFAP is detected in the CTX. (D) In the DG corresponding to the white square area in (A), AKT2 colocalizes with both ALDH1L1 and GFAP and is absent from the neuronal cell bodies in the granule cell layer. Lower panels: Higher magnification of the white square with profile views of the Z-stack images through an AKT2+ cell (arrow) confirms that AKT2 is present in the cell bodies of ALDH1L1+ and GFAP+ astrocytes. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34296180), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ALDH1L1 Antibody (2E7) - BSA Free

Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (2E7) - BSA Free [NBP2-50033] -

AKT2 is expressed in astroglia. (A) Representative image of AKT2 (red) costaining with astrocyte markers ALDH1L1 (green) and glial fibrillary acidic protein (GFAP, blue) in the HPC and CTX of coronal mouse brain sections. AKT2 is present throughout these brain regions, with strong immunoreactivity in the molecular layer of the DG. (B) Higher magnification of the rectangular area in (A) shows ALDH1L1 expression throughout cortical layers I-VI like AKT2, whereas GFAP is largely absent from the CTX and displays greater immunoreactivity in the HPC. (C) Higher magnification of the white square in (B) confirms that AKT2 colocalizes with ALDH1L1, while little to no GFAP is detected in the CTX. (D) In the DG corresponding to the white square area in (A), AKT2 colocalizes with both ALDH1L1 and GFAP and is absent from the neuronal cell bodies in the granule cell layer. Lower panels: Higher magnification of the white square with profile views of the Z-stack images through an AKT2+ cell (arrow) confirms that AKT2 is present in the cell bodies of ALDH1L1+ and GFAP+ astrocytes. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34296180), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ALDH1L1 Antibody (2E7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Simple Western

1:20 - 1:250

Western Blot

1:5000 - 1:10000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Mouse brain lysate, separated by Size, antibody dilution of 1;20, 1:100, 1:250, apparent MW was 91 kDa

Reviewed Applications

Read 1 review rated 3 using NBP2-50033 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH1L1

ALDH1L1 is encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.

Alternate Names

10-formyltetrahydrofolate dehydrogenase, aldehyde dehydrogenase 1 family, member L1, aldehyde dehydrogenase family 1 member L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, DKFZp781N0997, EC 1.5.1.6,10-FTHFDH, FDH, formyltetrahydrofolate dehydrogenase, FTHFD10-fTHF

Gene Symbol

ALDH1L1

UniProt

O75891 (Human)

Additional ALDH1L1 Products

Product Documents for ALDH1L1 Antibody (2E7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ALDH1L1 Antibody (2E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ALDH1L1 Antibody (2E7) - BSA Free

Customer Reviews for ALDH1L1 Antibody (2E7) - BSA Free (1)

3 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
100%
2 Stars
0%
1 Stars
0%

Have you used ALDH1L1 Antibody (2E7) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • ALDH1L1 Antibody (2E7)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: PFA-fixed cryosections of mouse brain
    Species: Mouse
    Verified Customer | Posted 07/19/2017
    Mouse hippocampus_CA1 area Green is aldh1l1 Red is GFAP
    Only works after antigen retrieval (citrate buffer, pH6; (sub)boiling for 10 min) Dilution 1:100
    ALDH1L1 Antibody (2E7) - BSA Free NBP2-50033

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies