ALDH1L1 Antibody (6H10Y3)

Novus Biologicals | Catalog # NBP3-16658

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6H10Y3 expressed in HEK293
View all ALDH1L1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 180-300 of human ALDH1L1 (O75891). VRLIAEGKAPRLPQPEEGATYEGIQKKETAKINWDQPAEAIHNWIRGNDKVPGAWTEACEQKLTFFNSTLNTSGLVPEGDALPIPGAHRPGVVTKAGLILFGNDDKMLLVKNIQLEDGKMI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH1L1 Antibody (6H10Y3)

Western Blot: ALDH1L1 Antibody (6H10Y3) [NBP3-16658]

Western Blot: ALDH1L1 Antibody (6H10Y3) [NBP3-16658]

Western Blot: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] - Western blot analysis of extracts of various cell lines, using ALDH1L1 Rabbit mAb (NBP3-16658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
ALDH1L1 Antibody (6H10Y3)

Immunohistochemistry: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] -

Immunohistochemistry: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] - Confocal imaging of Mouse liver using ALDH1L1 Rabbit mAb (dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 60x
ALDH1L1 Antibody (6H10Y3)

Immunohistochemistry-Paraffin: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] -

Immunohistochemistry-Paraffin: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] -Rat kidney using ALDH1L1 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ALDH1L1 Antibody (6H10Y3)

Immunohistochemistry-Paraffin: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] -

Immunohistochemistry-Paraffin: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] -Human liver using ALDH1L1 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ALDH1L1 Antibody (6H10Y3)

Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] -

Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] - Confocal imaging of Mouse liver using ALDH1L1 Rabbit mAb. DAPI was used for nuclear staining (blue). Objective: 60x.

Applications for ALDH1L1 Antibody (6H10Y3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ALDH1L1

ALDH1L1 is encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.

Alternate Names

10-formyltetrahydrofolate dehydrogenase, aldehyde dehydrogenase 1 family, member L1, aldehyde dehydrogenase family 1 member L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, DKFZp781N0997, EC 1.5.1.6,10-FTHFDH, FDH, formyltetrahydrofolate dehydrogenase, FTHFD10-fTHF

Gene Symbol

ALDH1L1

Additional ALDH1L1 Products

Product Documents for ALDH1L1 Antibody (6H10Y3)

Certificate of Analysis

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Product Specific Notices for ALDH1L1 Antibody (6H10Y3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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