ALDH5A1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86997

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ALDH5A1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RKWYNLMIQNKDDLARIITAESGKPLKEAHGEILYSAFFLEWFSEEARRVYGDIIHTPAKDRRALVLKQPIGVAAVITPWNFPSAM

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ALDH5A1 Antibody - BSA Free

Western Blot: ALDH5A1 Antibody [NBP1-86997]

Western Blot: ALDH5A1 Antibody [NBP1-86997]

Western Blot: ALDH5A1 Antibody [NBP1-86997] - Analysis using Anti-ALDH5A1 antibody NBP1-86997 (A) shows similar pattern to independent antibody NBP1-86996 (B).
Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-86997]

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-86997]

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-86997] - Staining in human liver and pancreas tissues using anti-ALDH5A1 antibody. Corresponding ALDH5A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-86997]

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-86997]

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-86997] - Staining of human liver shows high expression.
Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-86997]

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-86997]

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-86997] - Staining of human pancreas shows low expression as expected.

Applications for ALDH5A1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH5A1

ALDH5A1 belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

aldehyde dehydrogenase 5 family, member A1, EC 1.2.1, EC 1.2.1.24, NAD(+)-dependent succinic semialdehyde dehydrogenase, SSADHmitochondrial

Gene Symbol

ALDH5A1

Additional ALDH5A1 Products

Product Documents for ALDH5A1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ALDH5A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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