Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) - BSA Free

Novus Biologicals | Catalog # NBP1-74042

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6D10

Format

BSA Free
View all Aldo-keto Reductase 1C1/AKR1C1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Aldo-keto Reductase 1C1/AKR1C1 (1-323aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) - BSA Free

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10)BSA Free [NBP1-74042]

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10)BSA Free [NBP1-74042]

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) [NBP1-74042] - Lane 2. HepG2 cell lysate, Lane 3. Raji cell lysate.
Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) - BSA Free [NBP1-74042]

Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) - BSA Free [NBP1-74042]

Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) [NBP1-74042] - Analysis in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AKR1C1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) - BSA Free [NBP1-74042]

Flow Cytometry: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) - BSA Free [NBP1-74042]

Flow Cytometry: Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) [NBP1-74042] - Analysis of AKR1C1 in A431 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Applications for Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldo-keto Reductase 1C1/AKR1C1

The human aldo-keto reductases 1C1 and 1C3 (AKR1C1 and AKR1C3) have major roles in pre receptor regulation of progesterone action. They can both convert progesterone to the less potent efficiencies. AKR1C1 and AKR1C3 also act as 3-ketosteroid reductase, and as such they can convert the most potent androgen 5alpha-DHT into 3beta-andorstandiol, which is an estrogen receptor beta ligand, and into the inactive androgen 3alpha-androstnionl, respectively.

Long Name

Aldo-keto Reductase Family 1, Member C1

Alternate Names

2-ALPHA-HSD, 20-ALPHA-HSD, AKR1C1, AldoketoReductase 1C1, DDH1, HAKRC, HBAB

Gene Symbol

AKR1C1

UniProt

NP_001344 (Human)

Additional Aldo-keto Reductase 1C1/AKR1C1 Products

Product Documents for Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) - BSA Free

Certificate of Analysis

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Product Specific Notices for Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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