Aldo-keto Reductase 1C1/AKR1C1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-57667

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Aldo-keto Reductase 1C1/AKR1C1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: EPWVDPNSPVLLEDPVLCALAKKHK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aldo-keto Reductase 1C1/AKR1C1 Antibody - BSA Free

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody [NBP2-57667]

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody [NBP2-57667]

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody [NBP2-57667] - Analysis in human cell lines A-549 and A-431. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody [NBP2-57667]

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody [NBP2-57667]

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody [NBP2-57667] - Western blot analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1C1/AKR1C1 Antibody [NBP2-57667]

Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1C1/AKR1C1 Antibody [NBP2-57667]

Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C1/AKR1C1 Antibody [NBP2-57667] - Staining of human cell line Hep G2 shows localization to nucleus, nucleoli fibrillar center, cytosol & endoplasmic reticulum.

Applications for Aldo-keto Reductase 1C1/AKR1C1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldo-keto Reductase 1C1/AKR1C1

AKR1C1 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Long Name

Aldo-keto Reductase Family 1, Member C1

Alternate Names

2-ALPHA-HSD, 20-ALPHA-HSD, AKR1C1, AldoketoReductase 1C1, DDH1, HAKRC, HBAB

Gene Symbol

AKR1C1

Additional Aldo-keto Reductase 1C1/AKR1C1 Products

Product Documents for Aldo-keto Reductase 1C1/AKR1C1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Aldo-keto Reductase 1C1/AKR1C1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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