Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F9

Format

BSA Free
View all Aldolase A Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Aldolase A (1-364aa) purified from E. coli

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32681081).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Novus Biologicals Mouse Aldolase A Antibody (3F9) - BSA Free (NBP2-42620) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-Aldolase A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Aldolase A Antibody (3F9) - BSA Free

Western Blot: Aldolase A Antibody (3F9)BSA Free [NBP2-42620]

Western Blot: Aldolase A Antibody (3F9)BSA Free [NBP2-42620]

Western Blot: Aldolase A Antibody (3F9) [NBP2-42620] - The Recombinant Human ALDOA and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human ALDOA Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate
Immunocytochemistry/ Immunofluorescence: Aldolase A Antibody (3F9) - BSA Free [NBP2-42620]

Immunocytochemistry/ Immunofluorescence: Aldolase A Antibody (3F9) - BSA Free [NBP2-42620]

Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (3F9) [NBP2-42620] - Analysis of ALDOA in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human A549 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: Aldolase A Antibody (3F9) - BSA Free [NBP2-42620]

Flow Cytometry: Aldolase A Antibody (3F9) - BSA Free [NBP2-42620]

Flow Cytometry: Aldolase A Antibody (3F9) [NBP2-42620] - An intracellular stain was performed on HeLa cells with Aldolase A Antibody (3F9)NBP2-42620AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: Aldolase A Antibody (3F9) - BSA Free [NBP2-42620]

Flow Cytometry: Aldolase A Antibody (3F9) - BSA Free [NBP2-42620]

Flow Cytometry: Aldolase A Antibody (3F9) [NBP2-42620] - Analysis of ALDOA in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Applications for Aldolase A Antibody (3F9) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldolase A

Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Alternate Names

ALDA, aldolase A, fructose-bisphosphate, EC 4.1.2.13, fructose-1,6-bisphosphate triosephosphate-lyase, fructose-bisphosphate aldolase A, GSD12, Lung cancer antigen NY-LU-1, MGC10942, MGC17716, MGC17767, Muscle-type aldolase

Gene Symbol

ALDOA

UniProt

NP_908930 (Human)

Additional Aldolase A Products

Product Documents for Aldolase A Antibody (3F9) - BSA Free

Certificate of Analysis

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Product Specific Notices for Aldolase A Antibody (3F9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aldolase A Antibody (3F9) - BSA Free

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Protocols

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