Aldolase A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87488

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Aldolase A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aldolase A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Aldolase A Antibody [NBP1-87488]

Immunocytochemistry/ Immunofluorescence: Aldolase A Antibody [NBP1-87488]

Immunocytochemistry/Immunofluorescence: Aldolase A Antibody [NBP1-87488] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Aldolase A Antibody Aldolase A Antibody

Aldolase A Antibody [NBP1-87488] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Aldolase A Antibody [NBP1-87488] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Aldolase A Antibody Aldolase A Antibody

Aldolase A Antibody [NBP1-87488] -Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules.

Aldolase A Antibody [NBP1-87488] -Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules.
Aldolase A Antibody Aldolase A Antibody

Aldolase A Antibody [NBP1-87488] -Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.

Aldolase A Antibody [NBP1-87488] -Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
Aldolase A Antibody Aldolase A Antibody

Aldolase A Antibody [NBP1-87488] -Staining of human testis shows weak cytoplasmic positivity in Leydig cells.

Aldolase A Antibody [NBP1-87488] -Staining of human testis shows weak cytoplasmic positivity in Leydig cells.
Aldolase A Antibody Aldolase A Antibody

Aldolase A Antibody [NBP1-87488] -Analysis in human cell line U-87 MG.

Aldolase A Antibody [NBP1-87488] -Analysis in human cell line U-87 MG.

Applications for Aldolase A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldolase A

Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Alternate Names

ALDA, aldolase A, fructose-bisphosphate, EC 4.1.2.13, fructose-1,6-bisphosphate triosephosphate-lyase, fructose-bisphosphate aldolase A, GSD12, Lung cancer antigen NY-LU-1, MGC10942, MGC17716, MGC17767, Muscle-type aldolase

Gene Symbol

ALDOA

Additional Aldolase A Products

Product Documents for Aldolase A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Aldolase A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aldolase A Antibody - BSA Free

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Protocols

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FAQs

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