Alkaline Phosphatase/ALPP Antibody (H7E8) - BSA Free

Novus Biologicals | Catalog # NB500-532

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H7E8

Format

BSA Free
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Product Specifications

Immunogen

Purified placental alkaline phosphatase.

Localization

Attached to the membrane by a GPI-anchor.

Specificity

The antibody H7E8 recognizes throphoblast-derived membrane-associated enzyme alkaline phosphatase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Alkaline Phosphatase/ALPP Antibody (H7E8) - BSA Free

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (H7E8) - BSA Free [NB500-532]

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (H7E8) - BSA Free [NB500-532]

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (H7E8) [NB500-532] - Immunohistochemistry staining of human cerebellum (paraffin sections) using anti-Alkaline Phosphatase/PLAP (H7E8).

Applications for Alkaline Phosphatase/ALPP Antibody (H7E8) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1:1000
Application Notes
Predicted molecular weight: 58 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Alkaline Phosphatase/ALPP

Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.

Long Name

Alkaline Phosphatase, Placental Type

Alternate Names

Alkaline Phosphatase, PLAP, SEAP

Entrez Gene IDs

250 (Human)

Gene Symbol

ALPP

Additional Alkaline Phosphatase/ALPP Products

Product Documents for Alkaline Phosphatase/ALPP Antibody (H7E8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (H7E8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Alkaline Phosphatase/ALPP Antibody (H7E8) - BSA Free

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  • Q: I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?

    A:

    None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

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