alpha-Actinin 1 Antibody (OTI7A4)

Novus Biologicals | Catalog # NBP1-48251

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI7A4
View all alpha Actinin 1 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27512039). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Z-disk Marker

Specificity

This antibody is specific for Homo sapiens actinin, alpha 1 (ACTN1), transcript variant 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

102.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Actinin 1 Antibody (OTI7A4)

Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).
Immunocytochemistry/ Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunocytochemistry/ Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Western Blot: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha Actinin 1.
Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from 8 different cell lines by using anti-ACTN1 monoclonal antibody at 1:200 dilution.
Immunocytochemistry/ Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunocytochemistry/ Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Knockout Validated: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251]

Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, and ACTN1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ACTN1 monoclonal antibody (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
alpha-Actinin 1 Antibody (OTI7A4)

Simple Western:alpha-Actinin 1 Antibody (OTI7A4)[NBP1-48251]

Simple Western:alpha-Actinin 1 Antibody (OTI7A4)[NBP1-48251]Simple Western� analysis of endogenous protein Cytokeratin 8 from HELA lysates (0.5 mg/mL) using Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for alpha-Actinin 1 Antibody (OTI7A4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha Actinin 1

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

Long Name

Actinin alpha 1

Alternate Names

ACTN1, alpha-Actinin 1, alphaActinin 1, BDPLT15

Entrez Gene IDs

87 (Human)

Gene Symbol

ACTN1

Additional alpha Actinin 1 Products

Product Documents for alpha-Actinin 1 Antibody (OTI7A4)

Certificate of Analysis

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Product Specific Notices for alpha-Actinin 1 Antibody (OTI7A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Actinin 1 Antibody (OTI7A4)

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Protocols

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