Alpha Actinin 2 Antibody

Novus Biologicals | Catalog # NBP1-32462

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Zebrafish

Cited:

Mouse

Predicted:

Bovine (99%), Chicken (98%), Xenopus (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all alpha Actinin 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alpha Actinin 2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (94%).

Localization

Cytoplasm, myofibril, sarcomere, Z-disk

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alpha Actinin 2 Antibody

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse muscle. alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat muscle. alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. Alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat heart, alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Alpha Actinin 2 Antibody

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] -

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.
Blue: Hoechst 33342 staining.
Alpha Actinin 2 Antibody

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] -

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alpha Actinin 2 Antibody

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] -

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Alpha Actinin 2 Antibody

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] -

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Alpha Actinin 2 Antibody

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] -

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alpha Actinin 2 Antibody

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] -

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Alpha Actinin 2 Antibody

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] -

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Alpha Actinin 2 Antibody

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] -

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Alpha Actinin 2 Antibody

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] -

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alpha Actinin 2 Antibody

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] -

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Alpha Actinin 2 Antibody

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] -

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Alpha Actinin 2 Antibody

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] -

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alpha Actinin 2 Antibody

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Alpha Actinin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha Actinin 2

Alpha actinins are actin binding proteins that carry out different purposes in different different cell types. Skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN2 encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. There are several known transcript variants that result from the use of multiple polyA+ sites. ACTN2 has the ability to form both homo- and heterodimers (with ACTN3).

Long Name

Actinin alpha 2

Alternate Names

Actinin alpha 2, Actinin-a2, ACTN2, alpha-Actinin 2, Alpha-Actinin Skeletal Muscle, Alpha-Actinin-2, CMD1AA

Gene Symbol

ACTN2

Additional alpha Actinin 2 Products

Product Documents for Alpha Actinin 2 Antibody

Certificate of Analysis

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Product Specific Notices for Alpha Actinin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Alpha Actinin 2 Antibody

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Protocols

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