Alpha Actinin 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15355

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all alpha Actinin 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alpha Actinin 2. The exact sequence is proprietary.

Localization

Cytoplasm; myofibril; sarcomere; Z-disk

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alpha Actinin 2 Antibody - BSA Free

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355]

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355]

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] - Alpha Actinin 2 antibody detects Alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP2-15355]

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP2-15355]

Immunocytochemistry/Immunofluorescence: Alpha Actinin 2 Antibody [NBP2-15355] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP2-15355]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP2-15355]

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP2-15355] - Alpha Actinin 2 antibody detects Alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Alpha Actinin 2 antibody diluted at 1:250.
Western Blot: Alpha Actinin 2 Antibody [NBP2-15355]

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355]

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE, antibody diluted at 1:3000.
Western Blot: Alpha Actinin 2 Antibody [NBP2-15355]

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355]

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.
Alpha Actinin 2 Antibody

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] -

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] -Mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Alpha Actinin 2 Antibody

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP2-15355] -

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP2-15355] -Analysis of paraffin-embedded zebrafish tissue, using alpha Actinin 2 antibody [N2C1], Internal at 1:300 dilution.
Alpha Actinin 2 Antibody

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] -

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] - Sample (10 ug of whole cell lysate)
A: adult zebrafish
7.5% SDS PAGE
NBP2-15355 diluted at 1:3000

Applications for Alpha Actinin 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha Actinin 2

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Long Name

Actinin alpha 2

Alternate Names

Actinin alpha 2, Actinin-a2, ACTN2, alpha-Actinin 2, Alpha-Actinin Skeletal Muscle, Alpha-Actinin-2, CMD1AA

Gene Symbol

ACTN2

UniProt

P35609

Additional alpha Actinin 2 Products

Product Documents for Alpha Actinin 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Alpha Actinin 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for Alpha Actinin 2 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Alpha Actinin 2 Antibody - BSA Free and earn rewards!

Have you used Alpha Actinin 2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies