Alpha Actinin 4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15357

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Alpha Actinin 4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Alpha Actinin 4. The exact sequence is proprietary.

Localization

Nucleus; Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alpha Actinin 4 Antibody - BSA Free

Western Blot: Alpha Actinin 4 Antibody [NBP2-15357]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15357]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - Alpha Actinin 4 antibody detects ACTN4 protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract. 7.5% SDS-PAGE. Alpha Actinin 4 antibody dilution: 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody [NBP2-15357]

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody [NBP2-15357]

Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody [NBP2-15357] - Paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Western Blot: Alpha Actinin 4 Antibody [NBP2-15357]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15357]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Alpha Actinin 4 Antibody [NBP2-15357]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15357]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Alpha Actinin 4 Antibody [NBP2-15357]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15357]

Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract7.5 % SDS-PAGE dilution: 1:1000
Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357]

Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357]

Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357] - A549 xenograft, using alpha Actinin 4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357]

Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357]

Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357] - Mouse intestine. alpha Actinin 4 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Alpha Actinin 4 Antibody - BSA Free

Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 4 antibody [C2C3], C-term (NBP2-15357) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Alpha Actinin 4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

32 ug/mL

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Alpha Actinin 4

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]

Alternate Names

actinin alpha4 isoform, actinin, alpha 4, ACTININ-4, alpha-actinin-4, DKFZp686K23158, F-actin cross-linking protein, focal segmental glomerulosclerosis 1, FSGS, FSGS1, Non-muscle alpha-actinin 4

Gene Symbol

ACTN4

UniProt

O43707 (Human)

Additional Alpha Actinin 4 Products

Product Documents for Alpha Actinin 4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Alpha Actinin 4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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