Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25359

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1692 expressed in HEK293

Format

Azide and BSA Free
View all Alpha Actinin (pan) Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragmemt of human Alpha Actinin (pan)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free [NBP3-25359] -

Immunocytochemistry/Immunofluorescence: Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free [NBP3-25359] - alpha Actinin 2 antibody [HL1692] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [HL1692] (NBP3-25359) diluted at 1:500. Blue: Fluoroshield with DAPI.
Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

Western Blot: Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free [NBP3-25359] -

Western Blot: Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free [NBP3-25359] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin antibody [HL1692] (NBP3-25359) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

Immunohistochemistry-Paraffin: Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free [NBP3-25359] -

Immunohistochemistry-Paraffin: Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free [NBP3-25359] - alpha Actinin 2 antibody [HL1692] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. alpha Actinin 2 stained by alpha Actinin 2 antibody [HL1692] (NBP3-25359) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alpha Actinin (pan)

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins,including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple rolesin different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles andadherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, andsmooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillaractin filaments. This gene encodes a nonmuscle, cytoskeletal, alp

Alternate Names

actinin 1 smooth muscle, actinin, alpha 1, alpha-actinin 1, Alpha-actinin cytoskeletal isoform, alpha-actinin-1, F-actin cross-linking protein, FLJ40884, FLJ54432, Non-muscle alpha-actinin-1

Gene Symbol

ACTN1

Additional Alpha Actinin (pan) Products

Product Documents for Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free and earn rewards!

Have you used Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies