Alpha Fodrin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89460

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Alpha Fodrin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alpha Fodrin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Alpha Fodrin Antibody [NBP1-89460]

Immunocytochemistry/ Immunofluorescence: Alpha Fodrin Antibody [NBP1-89460]

Immunocytochemistry/Immunofluorescence: Alpha Fodrin Antibody [NBP1-89460] - Immunofluorescent staining of human cell line A-431 shows localization to microtubules & vesicles.
Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460] - Staining in human cerebral cortex and skeletal muscle tissues. Corresponding SPTAN1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody [NBP1-89460] - Staining of human kidney shows moderate cytoplasmic/membranous positivity.
Alpha Fodrin Antibody - BSA Free Western Blot: Alpha Fodrin Antibody - BSA Free [NBP1-89460]

Western Blot: Alpha Fodrin Antibody - BSA Free [NBP1-89460]

Analysis in human cell line HeLa.

Applications for Alpha Fodrin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alpha Fodrin

SPTAN1/Alpha II-spectrin is one of two polypeptide chains (alpha and beta) that are part of the fodrin tetramer. Fodrin functions to link actin filaments to the plasma membrane and is a major neuronal cytoskeletal protein. Fodrin has been linked to neurogenerative and autoimmune diseases.

Alternate Names

Alpha-II spectrin, EIEE5, FLJ17738, FLJ44613, Fodrin alpha chain, NEAS, spectrin alpha chain, brain, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Spectrin, non-erythroid alpha chain, SPTA2

Gene Symbol

SPTAN1

Additional Alpha Fodrin Products

Product Documents for Alpha Fodrin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Alpha Fodrin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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