Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Human, Mouse, Rat, Porcine, Bovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E3

Format

BSA Free
View all alpha-Internexin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant rat alpha Internexin expressed in and purified from E. coli [UniProt# P23565]

Epitope

The C-terminal non-helical extension of the protein.

Reactivity Notes

Porcine reported in scientific literature (PMID: 12197769).

Localization

Cytoplasmic

Marker

Immature Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Internexin Antibody (2E3) - BSA Free

Western Blot: alpha-Internexin Antibody (2E3) [NB300-140]

Western Blot: alpha-Internexin Antibody (2E3) [NB300-140]

Western Blot: alpha-Internexin Antibody (2E3) [NB300-140] - Analysis of different tissue lysates using alpha-Internexin, dilution 1:10000 (Red): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord lysate. The antibody reveals the alpha-Internexin protein with an apparent molecular weight of 64-66kDa, with some variability across species.
Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody (2E3) [NB300-140]

Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody (2E3) [NB300-140]

Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody (2E3) [NB300-140] - Hippocampal neurons in tissue culture with neurofilament alpha internexin (red), using NB300-140 and GFAP (green), using NB300-141.
Immunohistochemistry: alpha-Internexin Antibody (2E3) [NB300-140]

Immunohistochemistry: alpha-Internexin Antibody (2E3) [NB300-140]

Immunohistochemistry: alpha-Internexin Antibody (2E3) [NB300-140] - Section of rat facial nucleus 7 days following axotomy. These neurons are capable of regenerating their axons and also, concomitant with regeneration, strongly upregulate alpha-Internexin in their perikarya. Other central neurons which are not able to regenerate their axons do not upregulate this protein after axotomy and untreated facial neurons normally show only very low levels of alpha-Internexin. Both findings suggest that alpha-Internexin has a role in axonal regeneration.
Simple Western: alpha-Internexin Antibody (2E3) [NB300-140]

Simple Western: alpha-Internexin Antibody (2E3) [NB300-140]

Simple Western: alpha-Internexin Antibody (2E3) [NB300-140] - Simple Western lane view shows a specific band for alpha Internexin in 0.5 mg/ml of SH-SY5Y lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for alpha-Internexin Antibody (2E3) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:5000

Immunohistochemistry

1:5000

Immunohistochemistry-Paraffin

1:5000

Simple Western

1:200

Western Blot

1:10000
Application Notes
This alpha Internexin antibody is useful for Immunohistochemistry paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot. This antibody can be used on formalin-fixed cells in tissue culture and cryostat sections. A 66 kDa band can be seen in Western blotting. The epitope recognized by the 2E3 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of formalin-fixed paraffin-embedded histological sections.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in SH-SY5Y lysate, separated by Size, antibody dilution of 1:200, apparent MW was 140 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% PBS, 50% Glycerol

Format

BSA Free

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Internexin

In mammalian CNS, five key neuronal intermediate filament (NIF) proteins have been discovered which includes alpha-internexin (66 kD, INA or Inexa), peripherin (57 kD), and three neurofilament (NF) proteins, which are neurofilament light (NF-L, 68 kD), medium (NF-M, 145 kD), and heavy (NF-H, 200 kD). Among NIFs, alpha-internexin is categorized as class IV NIF which is highly expressed in most neurons during development especially when they begin to differentiate and before the expression of NF triplet proteins, and it is structurally/functionally associated with NF triplet proteins inside mature CNS neurons. Peripherin is mainly expressed in PNS and in some CNS neurons also, and alpha-internexin - peripherin self-assemble or co-assemble with neurofilament protein subunits to form filamentous structure before their translocation into the axons to become an essential constituent of shape-maintaining IF network in mature neurons. It has been recognized as one of the many proteins phosphorylated by ATM/ATR in response to DNA damage and has been associated with developmental, degenerative /overexpression causes neuronal cell death, and inflammatory processes of CNS. Abnormal NIF accumulation is a neuropathological signature of several neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, dementia with Lewy bodies, and amyotrophic lateral sclerosis. Alpha-internexin has been detected as a major component of pathological inclusions in frontotemporal dementia, also called 'neuronal intermediate filament inclusion disease (NIFID).

Alternate Names

alphaInternexin, INA, NEF5, NF-66, TXBP-1

Entrez Gene IDs

9118 (Human); 24503 (Rat)

Gene Symbol

INA

UniProt

P23565 (Rat)

Additional alpha-Internexin Products

Product Documents for alpha-Internexin Antibody (2E3) - BSA Free

Certificate of Analysis

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Product Specific Notices for alpha-Internexin Antibody (2E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Internexin Antibody (2E3) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for alpha-Internexin Antibody (2E3) - BSA Free

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  • Q: One of our customers bought the item NB300-140 (lot 9811) in November 2011. In February 2012, the ab was OK, but in March 2012 the antibody was coagulated in the vial. I think it's a contamination of the vial by any cause after opening, and we have no inquiry about a such observation by other customers. Did you have any inquiry about this antibody?

    A: I had a look at our stock, and it appears to be fine, though I did notice some precipitate in the bottom of the tubes. I am not sure what this is at this stage, but will run some gels to find out. It should not be cell debris, since the cells are not in the same chamber as the antibody rich supernatant in the CL350 flasks. It may be aggregates of antibody molecules and/or serum proteins. Those tissue culture supernatants can contain very large amounts of antibody, so it would not be too surprising if some antibody precipitated out. I would recommend that your customer centrifuge that material out in the meantime and use the supernatant for their experiments.

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