Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunoblotting

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H3.B8

Format

BSA Free
View all AlphaA Crystallin/CRYAA Primary Antibodies »

Product Specifications

Immunogen

Native Alpha Crystallin

Localization

Cytoplasm, Nucleus

Specificity

Detects approx 20kDa. Does not cross-react with aB-crystallin, Beta L-crystallin, BH- crystallin, gamma-crystallin, HSP25, HSP27 or HSP47 proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse AlphaA Crystallin/CRYAA Antibody (1H3.B8) - BSA Free (NBP2-12875) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-AlphaA Crystallin/CRYAA Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for AlphaA Crystallin/CRYAA Antibody (1H3.B8) - BSA Free

Western Blot: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875]

Western Blot: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875]

Western Blot: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Western Blot analysis of Bovine tissue lysate showing detection of AlphaA Crystallin/CRYAA protein using Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody, Clone 1H3.B8 (NBP2-12875). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody (NBP2-12875) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. This blot shows absolute specificity as Left: AlphaA Crystallin/CRYAA, Right: Alpha B Crystallin.
Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875]

Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875]

Immunocytochemistry/Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody, Clone 1H3.B8 (NBP2-12875). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody (NBP2-12875) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) AlphaA Crystallin/CRYAA Antibody. (D) Composite.
Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875]

Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875]

Immunocytochemistry/Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha A Crystallin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X.

Applications for AlphaA Crystallin/CRYAA Antibody (1H3.B8) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Application Notes
0.5 ug/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Alpha A Crystallin Antibody.Use in Immunoblotting reported in scientific literature (PMID 28546921)..

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AlphaA Crystallin/CRYAA

0.5ug/ml was sufficient for detection of 100ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.

Alternate Names

CRYA1, CRYAA, HSPB4

Gene Symbol

CRYAA

UniProt

NP_000385.1 (Human)

Additional AlphaA Crystallin/CRYAA Products

Product Documents for AlphaA Crystallin/CRYAA Antibody (1H3.B8) - BSA Free

Certificate of Analysis

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Product Specific Notices for AlphaA Crystallin/CRYAA Antibody (1H3.B8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

Citations for AlphaA Crystallin/CRYAA Antibody (1H3.B8) - BSA Free

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Protocols

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