AlphaA Crystallin/CRYAA Antibody
Novus Biologicals | Catalog # NB120-5595
![Western Blot: AlphaA Crystallin/CRYAA Antibody [NB120-5595]](https://resources.rndsystems.com/images/products/AlphaA-Crystallin-CRYAA-Antibody-Western-Blot-NB120-5595-img0004.jpg "Western Blot: AlphaA Crystallin/CRYAA Antibody [NB120-5595]")
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Key Product Details
Species Reactivity
Rat, Bovine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Purified alpha-A Crystallin from bovine lens.
Localization
Cytoplasmic
Specificity
This does not detect the unphosphorylated form of the protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AlphaA Crystallin/CRYAA Antibody
Western Blot: AlphaA Crystallin/CRYAA Antibody [NB120-5595]
Western Blot: AlphaA Crystallin/CRYAA Antibody [NB120-5595] - Analysis of alpha-A crystallin from bovine lens extract.Applications for AlphaA Crystallin/CRYAA Antibody
Application
Recommended Usage
Immunohistochemistry
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
0.1 ug/ml
Application Notes
WB: Detects an approx. 20 kDa representing alpha-A crystallin from bovine lens samples. IHC: Staining of alpha-A crystallin in rat thymus samples results in staining of epithelial reticular cells.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute to desired concentration.
Formulation
PBS with 30 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: AlphaA Crystallin/CRYAA
Additional AlphaA Crystallin/CRYAA Products
Product Documents for AlphaA Crystallin/CRYAA Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AlphaA Crystallin/CRYAA Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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