Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Rat IgG2A Clone # 206220
View all Amphiregulin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Amphiregulin
Ser94-Lys191
Accession # P31955

Specificity

Detects mouse Amphiregulin in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) Amphiregulin, rhEGF, rhHB-EGF, rhNRG-1 alpha, rhNRG-1-beta 1, rhTGF-alpha, recombinant mouse (rm) BTC, rmEpigen, or rmEpiregulin.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for Amphiregulin Antibody (206220) [CoraFluor™ 1]

Amphiregulin Antibody (206220) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for Amphiregulin Antibody (206220) [CoraFluor™ 1]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Amphiregulin

The protein encoded by the Amphiregulin gene is a member of the epidermal growth factor family. It is an autocrine growth factor aswell as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) andtransforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote thegrowth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encodedprotein is associated with a psoriasis-like skin phenotype. (provided by RefSeq)

Alternate Names

AREG

Gene Symbol

AREG

Additional Amphiregulin Products

Product Documents for Amphiregulin Antibody (206220) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Amphiregulin Antibody (206220) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

EGF Family

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Th2 Differentiation Pathway Th2 Differentiation Pathway Thumbnail