Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP2-68257

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.50 pg/mL

Assay Range

62.50-4000 pg/mL
View all AMPK alpha 1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

This kit recognizes Human AMPK in samples. No significant cross-reactivity or interference between Human AMPK and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 9.09%

Inter-Assay Precision (Precision between assays) CV% 10.61%

Recovery for Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

Recovery

89-113%

Linearity

Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

ELISA: Human AMPK alpha 1 ELISA Kit (Chemiluminescence) [NBP2-68257] -

ELISA: Human AMPK alpha 1 ELISA Kit (Chemiluminescence) [NBP2-68257] - Standard Curve Reference

Kit Contents for Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: AMPK alpha 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Gene Symbol

PRKAA1

Additional AMPK alpha 1 Products

Product Documents for Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Autophagy
Intracellular Kinases
Obesity

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