ANAPC2 Antibody (OTI1A6)

Novus Biologicals | Catalog # NBP2-01128

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1A6
View all ANAPC2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ANAPC2(NP_037498) produced in HEk293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

93.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ANAPC2 Antibody (OTI1A6)

Western Blot: ANAPC2 Antibody (1A6) [NBP2-01128] Analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Immunocytochemistry/ Immunofluorescence: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Immunocytochemistry/Immunofluorescence: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC2.
Immunohistochemistry-Paraffin: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Immunohistochemistry-Paraffin: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Human lymphoma tissue using anti-ANAPC2 mouse monoclonal antibody.
Flow Cytometry: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Flow Cytometry: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Flow Cytometry: ANAPC2 Antibody (1A6) [NBP2-01128] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANAPC2 antibody, and then analyzed by flow cytometry.
Western Blot: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Western Blot: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Western Blot: ANAPC2 Antibody (1A6) [NBP2-01128] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2.
Immunohistochemistry-Paraffin: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Immunohistochemistry-Paraffin: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ANAPC2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Immunohistochemistry-Paraffin: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ANAPC2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Immunohistochemistry-Paraffin: ANAPC2 Antibody (OTI1A6) [NBP2-01128]

Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ANAPC2 mouse monoclonal antibody.

Applications for ANAPC2 Antibody (OTI1A6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.74 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ANAPC2

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq].

Alternate Names

anaphase promoting complex subunit 2, anaphase-promoting complex subunit 2, APC2KIAA1406Cyclosome subunit 2, RP11-350O14.5

Entrez Gene IDs

29882 (Human)

Gene Symbol

ANAPC2

Additional ANAPC2 Products

Product Documents for ANAPC2 Antibody (OTI1A6)

Certificate of Analysis

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Product Specific Notices for ANAPC2 Antibody (OTI1A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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