Androgen R/NR3C4 Antibody (AN1-15) - BSA Free

Novus Biologicals | Catalog # NB300-615

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Gel Super Shift Assays

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG Clone # AN1-15

Format

BSA Free
View all Androgen R/NR3C4 Primary Antibodies »

Product Specifications

Immunogen

A fusion protein corresponding to residues 331-572 of human androgen receptor.

Localization

Nuclear

Specificity

Androgen receptor. This product does not cross-react with estrogen, progesterone or glucocorticoid receptors.

Clonality

Monoclonal

Host

Rat

Isotype

IgG

Scientific Data Images for Androgen R/NR3C4 Antibody (AN1-15) - BSA Free

Western Blot: Androgen R/NR3C4 Antibody (AN1-15)BSA Free [NB300-615]

Western Blot: Androgen R/NR3C4 Antibody (AN1-15)BSA Free [NB300-615]

Western Blot: Androgen R/NR3C4 Antibody (AN1-15) [NB300-615] - Analysis of 25 ug of T47D (lane 1), LNCaP (lane 2) and NIH-3T3 (lane 3) lysates.
Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (AN1-15) - BSA Free [NB300-615]

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (AN1-15) - BSA Free [NB300-615]

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (AN1-15) [NB300-615] - Analysis showing staining in the cytoplasm of human prostate tissue (B) and magnified section (C) compared with an isotype control (A).
Immunohistochemistry: Androgen R/NR3C4 Antibody (AN1-15) - BSA Free [NB300-615]

Immunohistochemistry: Androgen R/NR3C4 Antibody (AN1-15) - BSA Free [NB300-615]

Immunohistochemistry: Androgen R/NR3C4 Antibody (AN1-15) [NB300-615] - Immunolocalization of androgen receptor in primate brain.

Applications for Androgen R/NR3C4 Antibody (AN1-15) - BSA Free

Application
Recommended Usage

Gel Super Shift Assays

1:1 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Frozen

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Immunoprecipitation

1:10 - 1:500

Simple Western

1:100

Western Blot

1:50 - 1:500
Application Notes
WB: Detects an approx. 110 kDa protein representing androgen receptor in rat tissues. IHC: Staining of androgen receptor in primate hypothalamus results in intense nuclear staining.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with 50 ul sterilized PBS to a final concentration of 1.0 mg/ml.

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Androgen R/NR3C4

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the androgen receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain. The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Long Name

Androgen Receptor

Alternate Names

AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1

Entrez Gene IDs

367 (Human); 11835 (Mouse); 24208 (Rat)

Gene Symbol

AR

UniProt

P10275

Additional Androgen R/NR3C4 Products

Product Documents for Androgen R/NR3C4 Antibody (AN1-15) - BSA Free

Certificate of Analysis

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Product Specific Notices for Androgen R/NR3C4 Antibody (AN1-15) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Androgen R/NR3C4 Antibody (AN1-15) - BSA Free

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  • Q: What are the antibodies used to identify androgen receptors in brain ?

    A:

    I have carried out a search and I only see that NB300-615 has definitely been proven to detect Androgen receptors in the brain. Please see the fantastic image on the datasheet.

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail