Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free

Novus Biologicals | Catalog # NB110-85464

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Chicken

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0019-26B10

Format

Azide and BSA Free
View all Angiopoietin-1 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant human Angiopoietin-1

Reactivity Notes

Chicken reactivity reported from a verified customer review.

Specificity

No cross reactivity with human Angiopoietin-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Description

PLEASE NOTE: 0.02mg sample size is provided in reconstituted format and the 0.1mg size is provided lyophilized. (Please see reconstitution instuctions).

Scientific Data Images for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free

Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free [NB110-85464]

Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free [NB110-85464]

Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody (MM0019-26B10) [NB110-85464] - Angiopoietin 1 Antibody (MM0019-26B10) [NB110-85464] - Formalin fixed and paraffin embedded human placental tissue section is subjected to IHC with anti human Angiopoietin-1.

Applications for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:400

Immunohistochemistry-Paraffin

1:50-1:400

Western Blot

1:1000-1:2000
Application Notes
For use in FFPE tissue, our recommended antigen retrival-treat the paraffin section with 20ug/ml Proteinase K (in PBS) for 15-25min. Angiopoietin-1 antibody validated for IHC-FR from a verified customer review.

Reviewed Applications

Read 2 reviews rated 3 using NB110-85464 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 20 ug size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-1

Angiopoietin 1 is one of the Angiopoietin (Ang) family of growth factors. It binds and activates the TIE2 receptor tyrosine kinase by inducing its tyrosine phosphorylation. It is implicated in endothelial developmental processes later and distinct from that of VEGF. The structure of angiopoietin-1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Angiopoietin 1 appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It also mediates blood vessel maturation/stability and it may play an important role in the heart early development.

Alternate Names

ANGPT1

Entrez Gene IDs

284 (Human)

Gene Symbol

ANGPT1

UniProt

Q15389

Additional Angiopoietin-1 Products

Product Documents for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free

Customer Reviews for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free (2)

3 out of 5
2 Customer Ratings
5 Stars
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3 Stars
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Customer Images

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  • Name: Bryan Tinsley
    Application: Immunofluorescence
    Sample Tested:
    Species: Other
    Verified Customer | Posted 08/26/2014
    indirect immunofluorescence data with anti-Ang1 on chicken comb
    Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free NB110-85464
  • Name: Corey.
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen human skin sections
    Species: Human
    Verified Customer | Posted 07/12/2013
    Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free NB110-85464

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Protocols

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FAQs for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free

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  • Q: How do you dissolve the lyophilized Ab? Water or PBS? The datasheet for NB110-85464 just says "Buffer: Lyophilized" - - in Water? Buffer? Concentration of Buffer? NB110-85467 says Lyophilized from a solution of PBS - again how to dissolve the Ab - which PBS and what concentration?

    A: The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). 100 ug antibody is lyophilized from 40 ul of 1 x PBS. We suggest reconstituting the antibody with 500 ul sterile 1 x PBS (or to be perfect, with 40 ul H2O plus 460 ul PBS), to give a final concentration of 200 ug/ml. Alternatively, you may reconstitute the antibody in 40 ul H2O if a small volume is preferred.

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