Angiopoietin-like Protein 4/ANGPTL4 Antibody (Kairos4-397G) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80042
![Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody (Kairos4-397G)Azide and BSA Free [NBP2-80042]](https://resources.rndsystems.com/images/products/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Kairos4-397G-Western-Blot-NBP2-80042-img0002.jpg "Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody (Kairos4-397G)Azide and BSA Free [NBP2-80042]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # Kairos4-397G
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant human coiled-coil domain of Angiopoietin-like Protein 4/ANGPTL4.
Specificity
Recognizes the coiled-coil domain (CCD) of human Angiopoietin-like Protein 4/ANGPTL4. Detects a band of ~19kDa by Western blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images
Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody (Kairos4-397G)Azide and BSA Free [NBP2-80042]
Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody (Kairos4-397G) [NBP2-80042] - Western blot analysis using anti-ANGPTL4 (CCD) (human), mAb (Kairos4-397G) at 1:500 dilution. 1: Human ANGPTL4 (CCD) (FLAG(R)-tagged). 2: Human ANGPTL4 (FLD) (FLAG(R)-tagged). 3: Human ANGPTL4 (FLAG(R)-tagged). 4: Human RBP4 (FLAG(R)-tagged) (negative control). 5: Human ANGPTL3 (CCD) (FLAG(R)-tagged). 6: Human ANGPTL2 (CCD) (FLAG(R)-tagged). 7: Human ANGPTL5 (CCD) (FLAG(R)-tagged). 8: Human ANGPTL3 (FLAG(R)-tagged).![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody (Kairos4-397G) - Azide and BSA Free [NBP2-80042]](https://resources.rndsystems.com/images/products/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Kairos4-397G-Immunohistochemistry-Paraffin-NBP2-80042-img0001.jpg "Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody (Kairos4-397G) - Azide and BSA Free [NBP2-80042]")
Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody (Kairos4-397G) - Azide and BSA Free [NBP2-80042]
Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody (Kairos4-397G) [NBP2-80042] - Immunohistochemical staining of ANGPTL4 using anti-ANGPTL4 (coiled-coil domain) (human), mAb (Kairos4-397G) in human placenta tissue (10 ug/mL). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Applications
Application
Recommended Usage
ELISA
1:500 - 1:5000
Immunohistochemistry
10 ug/mL
Immunohistochemistry-Paraffin
10 ug/mL
Western Blot
1:500 - 1:5000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um-filtered solution in PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Angiopoietin-like Protein 4/ANGPTL4
Alternate Names
Angiopoietin like Protein 4, ANGPTL4, FIAF, HFARP, PGAR
Gene Symbol
ANGPTL4
Additional Angiopoietin-like Protein 4/ANGPTL4 Products
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- Angiopoietin-like Protein 4/ANGPTL4 Proteins and Enzymes
- Angiopoietin-like Protein 4/ANGPTL4 Simple Plex
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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