ANKRD5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85163

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ANKRD5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DCCKYIAQRGCDLKWKNLDHKTPRAVAKEGGFKAASKEIRRAERIANKLARPGAKNPNPLWALRLHDWSVEREAFLREAFAVLDRGDGSISKNDFVMVLE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANKRD5 Antibody - BSA Free

Western Blot: ANKRD5 Antibody [NBP1-85163]

Western Blot: ANKRD5 Antibody [NBP1-85163]

Western Blot: ANKRD5 Antibody [NBP1-85163] - Analysis in control (vector only transfected HEK293T lysate) and ANKEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ANKRD5 Antibody [NBP1-85163]

Immunocytochemistry/ Immunofluorescence: ANKRD5 Antibody [NBP1-85163]

Immunocytochemistry/Immunofluorescence: ANKRD5 Antibody [NBP1-85163] - Staining of human cell line A-431 shows positivity in nucleoli, plasma membrane and cytoplasm.
Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163]

Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163]

Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163]

Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163]

Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163] - Staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts and in Leydig cells.
Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163]

Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163]

Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163]

Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163]

Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163] - Staining of human fallopian tube shows strong positivity in cilia in glandular cells.
Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163]

Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163]

Immunohistochemistry-Paraffin: ANKRD5 Antibody [NBP1-85163] - Staining of human rectum shows moderate cytoplasmic and nuclear positivity in glandular cells.

Applications for ANKRD5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANKRD5

Alternate Names

ankyrin repeat domain 5, ankyrin repeat domain-containing protein 5, dJ839B4.6, FLJ21669

Entrez Gene IDs

63926 (Human)

Gene Symbol

ANKRD5

UniProt

Q9NU02 (Human)

Additional ANKRD5 Products

Product Documents for ANKRD5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ANKRD5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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