ANKRD53 Antibody (OTI1E1)
Novus Biologicals | Catalog # NBP2-00520
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI1E1
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-300 of human ANKRD53 (NP_079209) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ANKRD53 Antibody (OTI1E1)
Western Blot: ANKRD53 Antibody (1E1) [NBP2-00520] Analysis of extracts (35ug) from 9 different cell lines by using anti-ANKRD53 monoclonal antibody.
![Immunocytochemistry/ Immunofluorescence: ANKRD53 Antibody (OTI1E1) [NBP2-00520]](https://resources.rndsystems.com/images/products/ANKRD53-Antibody-1E1-Immunocytochemistry-Immunofluorescence-NBP2-00520-img0007.jpg "Immunocytochemistry/ Immunofluorescence: ANKRD53 Antibody (OTI1E1) [NBP2-00520]")
Immunocytochemistry/ Immunofluorescence: ANKRD53 Antibody (OTI1E1) [NBP2-00520]
Immunocytochemistry/Immunofluorescence: ANKRD53 Antibody (1E1) [NBP2-00520] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ANKRD53.![Immunohistochemistry-Paraffin: ANKRD53 Antibody (OTI1E1) [NBP2-00520]](https://resources.rndsystems.com/images/products/ANKRD53-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-00520-img0006.jpg "Immunohistochemistry-Paraffin: ANKRD53 Antibody (OTI1E1) [NBP2-00520]")
Immunohistochemistry-Paraffin: ANKRD53 Antibody (OTI1E1) [NBP2-00520]
Immunohistochemistry-Paraffin: ANKRD53 Antibody (1E1) [NBP2-00520] - Staining of paraffin-embedded Human lymphoma tissue using anti-ANKRD53 mouse monoclonal antibody.![Flow Cytometry: ANKRD53 Antibody (OTI1E1) [NBP2-00520]](https://resources.rndsystems.com/images/products/ANKRD53-Antibody-1E1-Flow-Cytometry-NBP2-00520-img0003.jpg "Flow Cytometry: ANKRD53 Antibody (OTI1E1) [NBP2-00520]")
Flow Cytometry: ANKRD53 Antibody (OTI1E1) [NBP2-00520]
Flow Cytometry: ANKRD53 Antibody (1E1) [NBP2-00520] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANKRD53 antibody, and then analyzed by flow cytometry.![Western Blot: ANKRD53 Antibody (OTI1E1) [NBP2-00520]](https://resources.rndsystems.com/images/products/ANKRD53-Antibody-1E1-Western-Blot-NBP2-00520-img0004.jpg "Western Blot: ANKRD53 Antibody (OTI1E1) [NBP2-00520]")
Western Blot: ANKRD53 Antibody (OTI1E1) [NBP2-00520]
Western Blot: ANKRD53 Antibody (1E1) [NBP2-00520] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53.![Immunohistochemistry-Paraffin: ANKRD53 Antibody (OTI1E1) [NBP2-00520]](https://resources.rndsystems.com/images/products/ANKRD53-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-00520-img0005.jpg "Immunohistochemistry-Paraffin: ANKRD53 Antibody (OTI1E1) [NBP2-00520]")
Immunohistochemistry-Paraffin: ANKRD53 Antibody (OTI1E1) [NBP2-00520]
Immunohistochemistry-Paraffin: ANKRD53 Antibody (1E1) [NBP2-00520] - Staining of paraffin-embedded Human lymph node tissue using anti-ANKRD53 mouse monoclonal antibody.![Flow Cytometry: ANKRD53 Antibody (OTI1E1) [NBP2-00520]](https://resources.rndsystems.com/images/products/ANKRD53-Antibody-1E1-Flow-Cytometry-NBP2-00520-img0001.jpg "Flow Cytometry: ANKRD53 Antibody (OTI1E1) [NBP2-00520]")
Flow Cytometry: ANKRD53 Antibody (OTI1E1) [NBP2-00520]
Flow Cytometry: ANKRD53 Antibody (1E1) [NBP2-00520] - Analysis of Hela cells, using anti-ANKRD53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).![Flow Cytometry: ANKRD53 Antibody (OTI1E1) [NBP2-00520]](https://resources.rndsystems.com/images/products/ANKRD53-Antibody-1E1-Flow-Cytometry-NBP2-00520-img0002.jpg "Flow Cytometry: ANKRD53 Antibody (OTI1E1) [NBP2-00520]")
Flow Cytometry: ANKRD53 Antibody (OTI1E1) [NBP2-00520]
Flow Cytometry: ANKRD53 Antibody (1E1) [NBP2-00520] - Analysis of Jurkat cells, using anti-ANKRD53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for ANKRD53 Antibody (OTI1E1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.58 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ANKRD53
Alternate Names
ankyrin repeat domain 53, ankyrin repeat domain-containing protein 53, FLJ12056, FLJ36160
Entrez Gene IDs
79998 (Human)
Gene Symbol
ANKRD53
Additional ANKRD53 Products
Product Documents for ANKRD53 Antibody (OTI1E1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ANKRD53 Antibody (OTI1E1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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