Ankyrin 3 Antibody (2A8) - BSA Free
Novus Biologicals | Catalog # NBP3-05528
![Western Blot: Ankyrin 3 Antibody [NBP3-05528]](https://resources.rndsystems.com/images/products/Ankyrin-3-Antibody-Western-Blot-NBP3-05528-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP3-05528]")
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2A8
Format
BSA Free
Loading...
Product Specifications
Immunogen
The C-terminal 398 amino acids of human Ankyrin 3 expressed in and purified from E. coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Ankyrin 3 Antibody (2A8) - BSA Free
Western Blot: Ankyrin 3 Antibody [NBP3-05528]
Western Blot: Ankyrin 3 Antibody [NBP3-05528] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody, at a at a dilution of of 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates. Bands at ~190kDa represent ankyrin 3 splice variants, higher molecular weight bands at 270kDa and 480kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.![Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528]](https://resources.rndsystems.com/images/products/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05528-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528]")
Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528]
Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.![Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528]](https://resources.rndsystems.com/images/products/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05528-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528]")
Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528]
Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.Applications for Ankyrin 3 Antibody (2A8) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry
1:1000
Western Blot
1:1000-1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50% PBS, 50% glycerol
Format
BSA Free
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: Ankyrin 3
Alternate Names
ankyrin G, Ankyrin G119, ankyrin-3, ankyrin-G, Node Of Ranvier
Gene Symbol
ANK3
Additional Ankyrin 3 Products
Product Documents for Ankyrin 3 Antibody (2A8) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ankyrin 3 Antibody (2A8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Ankyrin 3 Antibody (2A8) - BSA Free
There are currently no reviews for this product. Be the first to review Ankyrin 3 Antibody (2A8) - BSA Free and earn rewards!
Have you used Ankyrin 3 Antibody (2A8) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...